Skip to main content

In vitro propagation of Catalpa ovata G. Don


Plants of C. ovata were regenerated in vitro from shoot tips and nodal explants as well as from cotyledon-derived calluses. For shoot proliferation from shoot tips and nodal segments, Schenk and Hildebrandt (1972) or Lloyd and McCown (1980) basal media, supplemented with 6-benzyladenine (2.2–22.2 μM) alone or in combination with indole-3-acetic acid (0.6 μM), were used. Shoot regeneration through organogenesis was achieved by culturing cotyledons on Schenk and Hildebrandt medium containing indole-3-acetic acid (0.6 μM) and 6-benzyladenine (4.4 μM) or zeatin (22.8 μM). TLC and HPLC analysis showed that the multiple shoots and micropropagated plants exhibited similar iridoid patterns as those of the leaves of original plants of C. ovata. The highest levels of catalpol and catalposide (8.2 and 2.4 % of dry weight, respectively) were found in aerial parts of three-month-old in vitro regenerated plants.

This is a preview of subscription content, access via your institution.


  1. Bugala W (1991) Rodzaj Catalpa L. In: Drzewa i Krzewy (pp. 558–561). PWRiL, Warszawa

    Google Scholar 

  2. Huetteman CA & Preece JE (1993) Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119

    CAS  Article  Google Scholar 

  3. Ikeshima S & Uchida K (1936) The diuretic action of Catalpa seed. Jpn. J. Med. Sci. IV Pharmacol. Trans. 9: 37

    CAS  Google Scholar 

  4. Inoue K, Shiobara Y, Chen CC, Sakuyama S & Inouye H (1979) Quinones and related compounds in higher plants. VII. Supplementary studies on the constituents of the wood of Catalpa ovata. Yakugaku Zasshi 99: 500–504

    PubMed  CAS  Google Scholar 

  5. Koriyone T & Kimura Y (1962) Japanese and Chinese medicinal plants In: Hirshman JL, Ma TS (eds) Microchemical Investigation of Medicinal Plants. III. The Seeds of Catalpa Tree (pp. 262–271). Microchem. Acta, Vienna

    Google Scholar 

  6. Lloyd G & McCown B (1980) Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot-tip culture. Comb. Proc. Int. Plant Prop. Soc. 30: 421–427

    Google Scholar 

  7. Long C, Maulis C, Stanislas E & Fouraste I (1995) L'aucuboside et catalpol dans les feuilles de Plantago lanceolata L., Plantago major L., et Plantago media L. J. Pharm. Belg. 50: 484–488

    CAS  Google Scholar 

  8. Manojlovi? M, Vinterhalter B & Vinterhalter D (1994) In vitro propagation of Catalpa ovata G. Don and Catalpa bignonioides Walt. Glas. Inst. Bot. I Baste Univ. U Beogradu 26–27: 7–l4

    Google Scholar 

  9. Murashige T & Skoog F (1962) A revised medium for rapid growth bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497

    CAS  Article  Google Scholar 

  10. Okuda T, Yoshida T & Ono I (1975) Two new flavone glycosides from Catalpa ovata. Phytochemistry 14: 1654–1656

    CAS  Article  Google Scholar 

  11. Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction growth monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204

    CAS  Google Scholar 

  12. Suzuki Y, Hajime F & Ikko Y (1968) Diuretic action of the fruit of Catalpa ovata G. Don. Folia Pharmacol. Jpn. 64: 93–l07

    CAS  Google Scholar 

  13. Suzuki H, Murakami M, Yashizaki M & Havada M (1991) Preparation of Catalpa fruits and changes in the contents of catalposide, catalpol and p-hydroxybenzoic acid. Iyakuhin Kenkyu 22: 359–367

    CAS  Google Scholar 

  14. Upadhyaya S & Chandra N (1983) Shoot and plantlets formation in organ and callus cultures of Albizia lebbeck Benth. Ann. Bot. 50: 421–424

    Google Scholar 

  15. Wysoki?ska H & ?wiatek L (1989) Micropropagation of Catalpa bignonioides Walt. through tissue cultures. Acta Soc. Bot. Pol. 58: 185–198

    Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Halina Wysokinska.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lisowska, K., Wysokinska, H. In vitro propagation of Catalpa ovata G. Don. Plant Cell, Tissue and Organ Culture 60, 171–176 (2000).

Download citation

  • Bignoniaceae
  • Catalpa ovata
  • catalpol
  • catalposide
  • micropropagation
  • organogenesis