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Micropropagation of Chokecherry and Pincherry cultivars

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Abstract

In vitro culture establishment, shoot proliferation and ex vitro rooting responses of chokecherry (Prunus virginiana L.), `Garrington', and pincherry (P. pensylvanica L.f), `Mary Liss' and `Jumping Pound', were examined using various combinations of growth regulators. Dormant winter buds were used as explants. MSMO medium supplemented with 0.49 μM IBA and either 4.44 or 8.87 μM BA was found to be optimal for culture initiation of both species and cultivars. GA3 (28.89 μM) significantly reduced (p=0.0001) the number of successfully established cultures. BA concentrations 8.87–12.82 μM gave optimal shoot proliferation in chokecherry and 4.44 μM BA in both cultivars of pincherry. Auxin treatments were required for ex vitro rooting of approximately 10 mm long shoots in peat/perlite (1:1 v/v) mixture, at 25 °C, under mist. The best rooting (84%) was obtained with IBA/NAA (9.80/2.69 μM). A commercial rooting powder, Rootone F, containing IBA/NAA (0.057/0.067%) mixture, was also effective (75%). The ex vitro rooted plantlets did not require any additional acclimatization prior to transplanting to the regular greenhouse conditions.

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Authors and Affiliations

  1. Crop Diversification Centre North, 17507-, Fort Road, Edmonton, AB, Canada, T5B 4K3

    Kris W. Pruski, Kris W. Pruski & Tina Lewis

  2. Department of Plant Science, Nova Scotia Agricultural College, P.O. Box 550, Truro, NS, Canada, B2N 5E3

    Kris W. Pruski & Jerzy Nowak

  3. Department of Mathematics, Physics and Humanities, Canada

    Tess Astatkie

Authors
  1. Kris W. Pruski
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  2. Tina Lewis
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  3. Tess Astatkie
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  4. Jerzy Nowak
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Correspondence to Kris W. Pruski.

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Pruski, K.W., Lewis, T., Astatkie, T. et al. Micropropagation of Chokecherry and Pincherry cultivars. Plant Cell, Tissue and Organ Culture 63, 93–100 (2000). https://doi.org/10.1023/A:1006461423373

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  • DOI: https://doi.org/10.1023/A:1006461423373

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