Skip to main content

Direct regeneration of shoots from immature inflorescence cultures of turmeric

Abstract

Plant regeneration from cultured immature inflorescence of Curcuma longa was obtained by direct shoot development on Murashige and Skoog's basal medium supplemented with BA (5 or 10 mg l−1) in combination with 2,4-D (0.2 mg l−1) or NAA (0.1 mg l−1) and TDZ (1 or 2 mg l−1) in combination with IAA (0.1 mg l−1). Regenerated shoots were grown on MS medium for further development and later transferred to medium supplemented with 0.1 mg l−1 NAA for induction of roots. Complete plants thus obtained were transferred to sterilized soil in paper cups for 3–4 weeks and then to field, where 95% of the plants survived to maturity.

This is a preview of subscription content, access via your institution.

References

  1. Babu KN, Samsudeen K & Ravindran PN (1992) Direct regeneration of plantlets from immature inflorescence of ginger (Zingiber officinale Rosc.) by tissue culture. J. Spic Arom. Crops 1: 43–48

    Google Scholar 

  2. Balachandran SM, Bhat SR & Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8: 521–524

    CAS  Article  Google Scholar 

  3. Eapen S & George L (1997) Plant regeneration from peduncle segments of oil seed Brassica species: Influence of silver nitrate and silver thiosulphate. Plant Cell Tiss. Org. Cult. 51: 229–232

    CAS  Article  Google Scholar 

  4. Eapen S & Rao PS (1985) Plant regeneration from immature inflorescence callus cultures of wheat, rye and triticale. Euphytica 34: 153–159

    Article  Google Scholar 

  5. Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension culture of soyabean cells. Exp. Cell. Res. 50: 150–158

    Article  Google Scholar 

  6. George EF & Sherrington AD (eds) (1984) Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories (55 pp). Eastern Press, Reading, Berks

  7. Mok MC, Mok DWS, Turner JE & Mujer CV (1987) Biological and biochemical effect of cytokinin active phenylurea derivatives in tissue culture system. HortScience 22: 1194–1197

    CAS  Google Scholar 

  8. Mukhri Z & Yamaguchi H (1986) In vitro plant multiplication from rhizomes of turmeric (Curcuma domestica val) and Temoe Lawak (C. xanthorhiza Robx.). Plant Tiss. Culture Lett. 3(1): 28–30

    Google Scholar 

  9. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 473–497

    CAS  Article  Google Scholar 

  10. Murthy BNS, Murch SJ & Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In vitro Cell Dev. Biol. Plant 34: 267–275

    CAS  Google Scholar 

  11. Nadgauda RS, Mascarenhas AF, Hendre RR & Jagannathan V (1978) Rapid multiplication of turmeric (Curcuma longa L.). Indian J. Exp. Biology 16: 120–122

    CAS  Google Scholar 

  12. Satyavati GV, Raina MK & Sharma M (eds) (1976) Medicinal Plants of India, Vol. 1 (pp 312–317). Indian Council of Medical Research, New Delhi

    Google Scholar 

  13. Sit AK & Tiwari RS (1997) Micropropagation of turmeric (Curcuma longa L.). Rec. Hortic. 4: 145–148

    Google Scholar 

  14. Vasil IK (1982) Plant cell culture and somatic cell genetics of cereals and grasses. In: Vasil IK, Scowcroft WR & Frey KJ (eds) Plant Improvement and Somatic Cell Genetics. (pp 179–203). Academic Press, New York

    Google Scholar 

  15. Wannakrairoj S (1997) Clonal micropropagation of patumma (Curcuma alismatifolia Gagnep). Kas. J. Nat. Sci. 31: 353–356

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Leela George.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Salvi, N.D., George, L. & Eapen, S. Direct regeneration of shoots from immature inflorescence cultures of turmeric. Plant Cell, Tissue and Organ Culture 62, 235–238 (2000). https://doi.org/10.1023/A:1006459822879

Download citation

  • Curcuma longa L.
  • immature inflorescence
  • shoot regeneration
  • turmeric
  • Zingiberaceae