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Effect of 2,4-D and light quality on callus production and differentiation from in vitro cultured quince leaves

Abstract

The effects of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration, length of induction period and light quality on leaf regeneration of quince clone BA 29 were investigated. After 2, 4 or 6 days of induction with 2.5 mg l−1 or 5.0 mg l−1 2,4-D, leaves were cultured under red, blue, red+blue, far-red+blue, white, far-red light or darkness conditions. Leaves thereby treated showed different responses, with respect to somatic embryogenesis, callus, red-nodular structures or roots. Callus production increased with increasing 2,4-D concentration and induction period, although it was not influenced by light quality; the only exception was far-red+blue light, which reduced callusing response. This result suggested involvement of the blue-absorbing photoreceptor system in the callus formation processes. A high regeneration of red-nodular structures with a meristematic appearance was also observed; from some histological characterizations, we presumed they were adventitious buds that were arrested at an early developmental stage. Red-nodular structures increased with decreasing 2,4-D concentration and induction period. In the regeneration of such structures, the blue-absorbing photoreceptor system appeared to have a negative effect but only at a low photoequilibrium value. In contrast, light quality which activated phytochrome induced an increment in regeneration, but the response did not vary for photoequilibrium values ranging from 0.43 to 0.86. For root regeneration, phytochrome seemed to be the only photoreceptor involved.

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Morini, S., D'Onofrio, C., Bellocchi, G. et al. Effect of 2,4-D and light quality on callus production and differentiation from in vitro cultured quince leaves. Plant Cell, Tissue and Organ Culture 63, 47–55 (2000). https://doi.org/10.1023/A:1006456919590

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  • DOI: https://doi.org/10.1023/A:1006456919590

  • Cydonia oblonga
  • photoreceptors