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Effect of 2,4-D and light quality on callus production and differentiation from in vitro cultured quince leaves


The effects of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration, length of induction period and light quality on leaf regeneration of quince clone BA 29 were investigated. After 2, 4 or 6 days of induction with 2.5 mg l−1 or 5.0 mg l−1 2,4-D, leaves were cultured under red, blue, red+blue, far-red+blue, white, far-red light or darkness conditions. Leaves thereby treated showed different responses, with respect to somatic embryogenesis, callus, red-nodular structures or roots. Callus production increased with increasing 2,4-D concentration and induction period, although it was not influenced by light quality; the only exception was far-red+blue light, which reduced callusing response. This result suggested involvement of the blue-absorbing photoreceptor system in the callus formation processes. A high regeneration of red-nodular structures with a meristematic appearance was also observed; from some histological characterizations, we presumed they were adventitious buds that were arrested at an early developmental stage. Red-nodular structures increased with decreasing 2,4-D concentration and induction period. In the regeneration of such structures, the blue-absorbing photoreceptor system appeared to have a negative effect but only at a low photoequilibrium value. In contrast, light quality which activated phytochrome induced an increment in regeneration, but the response did not vary for photoequilibrium values ranging from 0.43 to 0.86. For root regeneration, phytochrome seemed to be the only photoreceptor involved.

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  • Antonelli M (1995) The regenerative ability of Quince BA29 in vitro. Adv. Hort. Sci. 9: 3–6

    Google Scholar 

  • Baker BS & Bhatia SK (1993) Factors affecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga). Plant Cell Tiss. Org. Cult. 35: 273–277

    CAS  Article  Google Scholar 

  • Chi GL & Pua EC (1989) Effect of ethylene inhibitors on high frequency plant regeneration from cotyledons of Brassica campestris sspp. chinensis in vitro. Proc. Moet-Hennessy Conference Maitrise de la Morphogenèse, Aix les Bains

  • D'Onofrio C, Morini S & Bellocchi G (1998) Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell Tiss. Org. Cult. 53: 91–98

    Article  Google Scholar 

  • D'Onofrio C, Morini S & Vitagliano C (1999) Isolation of protoplasts from in vitro-grown quince BA 29 leaves. In Vitro Cell. Dev. Biol. Plant 35: 421–423

    Google Scholar 

  • Dolcet-Sanjuan R, Mok DWS & Mok MC (1991) Plantlet regeneration from cultured leaves of Cydonia oblonga L. (quince). Plant Cell Rep. 10: 240–242

    Article  Google Scholar 

  • Fuernkranz HA, Nowak CA & Maynard CA (1990) Light effects on in vitro adventitious root formation in axillary shoots of mature Prunus serotina. Physiol. Plant. 80: 337–341

    Article  Google Scholar 

  • Galston AW & Baker RS (1949) Studies on the physiology of light action. II The photodynamic action of riboflavin. Am. J. Bot. 36: 773–780

    Article  Google Scholar 

  • Helsel DR & Hirsch RM (1995) Statistical Methods in Water Resources. Studies in Environmental Science: 49. Elsevier, Amsterdam

    Google Scholar 

  • Kraepiel Y & Miginiac E (1997) Photomorphogenesis and phytohormones. Plant Cell Environ. 20: 807–812

    CAS  Article  Google Scholar 

  • Mancinelli AL (1995) The physiology of phytochrome action. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in Plants. 2nd edition (pp 211–219)

  • Mol J, Jenkins G, Schafer E & Weiss D (1996) Signal perception, trasduction, and gene expression involved in anthocyanin biosynthesis. Crit. Rev. Plant Sci. 15: 525–557

    CAS  Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    CAS  Article  Google Scholar 

  • Roustan JP, Latche A & Fallot J (1989) Control of carrot somatic embryogenesis by inhibitors of ethylene synthesis or ethylene action. Proc. Moet-Hennessy Conference Maitrise de la Morphogenèse, Aix les Bains

  • Schneider H (1981) Plant anatomy and general botany. In: Clark G (ed) Staining procedures. 4th edition (pp 315–333).Williams and Wilkins Press, Baltimore, USA

    Google Scholar 

  • Song PS (1987) Possible primary photoreceptor. In: H Senger (ed) Blue Light Responses: Phenomena and Occurence in Plants and Microorganisms, Vol. II (pp 3–17). CRC Press, Inc., Boca Raton, FL.

    Google Scholar 

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Morini, S., D'Onofrio, C., Bellocchi, G. et al. Effect of 2,4-D and light quality on callus production and differentiation from in vitro cultured quince leaves. Plant Cell, Tissue and Organ Culture 63, 47–55 (2000).

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  • Cydonia oblonga
  • photoreceptors