Abstract
High yields of protoplasts were isolated from litchi embryogenic suspensions, which were maintained by alternative culture in liquid and on solid media containing silver thiosulfate. Protoplasts in liquid culture and agarose beads were unable to divide sustainedly, whereas embedding of protoplasts in Ca-alginate supported cell division to microcalli and the direct formation of somatic embryos from protoplasts. Nurse cells of litchi further enhanced the culture efficiency when protoplasts were cultured in Ca-alginate beads. White non-hyperhydric somatic embryos were developed from protoplast-derived microcalli or proembryos, and 33.1% of white somatic embryos regenerated into plantlets.
Similar content being viewed by others
References
Damm B & Willmitzer L (1988) Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes. Mol. Gen. Genet. 213: 15–20
Frearson EM, Power JB & Cocking EC (1973) The isolation, culture, and regeneration of Petunia leaf protoplasts. Dev. Biol. 33: 130–137
Fu L & Tang D (1983) Introduction pollen plants of litchi tree (Litchi chinensis Sonn.). Acta Genetica Sinica 10: 369–374
Gamborg OL, Miller RA & Ojima K (1968) Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Huancaruna-Perales E & Schieder O (1993) Plant regeneration form leaf protoplasts of apple. Plant Cell. Tiss. Org. Cult. 34: 71–76.
Kantharajah, AS, McConchie CA & Dodd WA (1992) In vitro embryo culture and induction of multiple shoots in lychee (Litchi chinensis Sonn.). Ann. Bot. 70: 153–156
Kao KN & Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110
Li ZY, Tanner GJ & Larkin PJ (1990) Callus regeneration from Trifolium subterraneum protoplasts and enhanced protoplast di58 vision by low-voltage treatment and nurse cells. Plant Cell Tiss. Org. Cult. 21: 67–73
Li ZY, Xia GM & Chen HM (1992) Somatic embryogenesis and plant regeneration from protoplasts isolated from embryogenic cell suspensions of wheat (Triticum aestivum L.). Plant Cell Tiss. Org. Cult. 28: 79–85
Menzel CM (1985) Propagation of lychee: A review. Sci. Hortic. 25: 31–48
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioasays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Niedz RP (1993) Culturing embryogenic protoplasts of 'Hamlin' sweet orange in calcium alginate beads. Plant Cell Tiss. Org. Cult. 34: 19–25
Nyman M & Wallin A (1992) Improved culture technique for strawberry (Fragaria x anannassa Duch.) protoplasts and the determination of DNA content in protoplast derived plants. Plant Cell Tiss. Org. Cult. 30: 127–133
Schnabl H, Youngman RJ & Zimmerman U (1983) Maintenance of plant cell membrane integrity and function by the immobilization of protoplasts in alginate matrices. Planta 158: 392–397
Teasdale RD & Richards DK (1991) Study of a factor produced by suspension cultured Pinus radiata cells which enhances cell growth at low inoculum densities. Plant Cell Tiss. Org. Cult. 26: 53–59
Walters TW & Earle ED (1990) A simple, versatile feeder layer system for Brassica oleracea protoplast culture. Plant Cell Rep. 9: 316–319
Witjaksono, Litz RE & Grosser JW (1998) Isolation, culture and regeneration of avocado (Persea americana Mill.) protoplasts. Plant Cell Rep. 18: 235–242
Yu CH (1997) Studies on protoplast culture of litchi (Litchi chinensis). PhD dissertation, Fujian Agricultural University, Fuzhou, China
Yu CH & Chen ZG (1997) Induction of litchi embryogenic calli by immature embryos and anthers culture in vitro. J. Fujian Agri. Univ. 26: 168–172 (in Chinese with English abstract)
Yu CH & Chen ZG (1998) Embryogenic suspension culture and protoplast isolation in litchee. Chinese J Trop Crops 19: 16–20 (in Chinese with English abstract)
Zhou LN, Kuang ZS, Ma XJ, Chen JQ & Cao J (1996) The study of factors affecting somatic embryogenesis in young embryo culture of Litchi chinensis. J Agric Biotech. 4: 161–165 (in Chinese with English abstract)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yu, C., Chen, Z., Lu, L. et al. Somatic embryogenesis and plant regeneration from litchi protoplasts isolated from embryogenic suspensions. Plant Cell, Tissue and Organ Culture 61, 51–58 (2000). https://doi.org/10.1023/A:1006446506041
Issue Date:
DOI: https://doi.org/10.1023/A:1006446506041