Skip to main content
SpringerLink
Log in

In vitro propagation of a semi-dwarfing cherry rootstock

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

A successful in vitro propagation system for the semi-dwarfing cherry rootstock Maxma-14 (Prunus avium L.) has been developed. Shoot tips and axillary buds were successfully established in vitro. Multiplication rate of about 6 was achieved over a 4-week period using Murashige and Skoog medium with 4.44 μM benzyladenine and 0.49 μM indole-3-butyric acid (IBA). Rooting occurred within 4 weeks on liquid and agar-gelled media containing 0.49 μM NAA or 0.49, 2.45 μM IBA. On liquid media, a maximum rooting efficiency of up to 100% was obtained. However, high concentrations of auxins delayed the time of root initiation for 3–5 days. Acclimatization was affected directly by rooting conditions. Survival was best when plantlets were transferred to pots after a short period of root emergence on rooting media. Multiplication medium was also important for successful acclimatization. Shoots transferred to rooting media from that with kinetin resulted in better acclimatization and survival than that derived from media with benzyladenine. Further, plantlets rooted on liquid media had better survival than that rooted on agar-gelled media.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abbott JA & Whitely E (1976) Cultures of Malus tissues in vitro. I. Multiplication of apple plants from isolated shoot apieces. Scientia Hort. 4: 183–189

    Article  Google Scholar 

  • Aldwinckle HS & Buturac I (1981) In vitro culture of grapevine for study of obligate pathogenes. Environ. Exp. Bot. 21 (3/4): 439

    Google Scholar 

  • Bergman L, Arnold SV & Erickson T (1985) Effect of N6-benzyladenine on shoot of five willow clones (Salix spp.) cultured in vitro. Plant Cell Tiss. Org. Cult. 4: 135–144

    Article  Google Scholar 

  • Boxus Ph & Terzi JM (1988) Control of accidential contamination during mass propagation. Acta Hort. 225: 189–191

    Google Scholar 

  • Druart P (1980) Plantlet regeneration from root callus of different Prunus specis. Scientia Hort. 12: 339–342

    Article  CAS  Google Scholar 

  • Edin M, Garcin A, Lichou J & Jourdain JM (1996) Influence of dwarfing cherry rootstocks on fruit production. Proc. Intl. Cherry Symposium. Acta Hort. 410: 507–510

    Google Scholar 

  • Gayner JA, Jones OP, Watkins R & Hopgood ME (1980) Regeneration of plants from callus. Rep. East Malling Res. Stn for 1979 (pp 187–188)

  • Hartman HT & Kester DE (1983) Plant Propagation, Principles and Practices. 4th edition. Prentice-Hall, Englewood Cilffs, NJ

    Google Scholar 

  • Hrotkó K & Fuzessery A (1996) Effect of rootstock on the branching and quality of cherry trees in the nursery. Acta Hort. 410: 507–510

    Google Scholar 

  • Hrotkó K & Simon G (1996) Effect of rootstock on the growth and productivity of cherry trees. Acta Hort. 410: 519–525

    Google Scholar 

  • Hrotko K, Magyar L, Simon G & Hanusz B (1997) Effect of rootstocks and interstocks on growth and yield of sweet cherry trees. Acta Hort. 451: 231–236

    Google Scholar 

  • James DJ, Passey AJ & Malhotra SB (1984) Organogenesis in callus derived from stem and leaf tissues of apple and cherry rootstocks. Plant Cell Tiss. Org. Cult. 3: 333–341

    Article  CAS  Google Scholar 

  • Jones OP & Hopgood ME (1979) The successful propagation in vitro of two rootstocks of Prunus: the plum rootstock Pixy (P.insititia) and the cherry rootstock F12/1 (P.avium). J. Hort. Sci. 54: 63–66

    Google Scholar 

  • Kappel F (1993) Sweet cherry management in Europe. Compact Fruit Tree. 26: 123–124

    Google Scholar 

  • Kappel F & Lichou J (1994) Flowering and fruiting of Burlat sweet cherry on size-controlling rootstock. HortScience 29(6): 611–612

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant. 15: 473–479

    Article  CAS  Google Scholar 

  • Németh G (1979) Benzyladenine-stimulated rooting in fruit-tree rootstocks cultured in vitro. Z. Pflanzenphysiol Bd. 95, S. 381–388

    Google Scholar 

  • Németh G (1981) Adventitoud root induction by substituted 2-chloro-3-phenylpropionnitrilies in apple rootstock cultured in vitro. Z. Pflanzenphysiol 95: 389–396

    Google Scholar 

  • Nordstrom AC & Eliasson L (1986) Uptake and translocation of C14-labled benzylaminopurine in apple shoots grown in vitro in relation to shoot development. Physiol Plantarium 68(3): 431–435

    Article  Google Scholar 

  • Pevalek-Kozlina B & Jelaska S (1987) Microclonal propagation of Prunus avium. Acta Hort. 212: 599–601

    Google Scholar 

  • Svensen SE (1991) Rooting and lateral shoot elongation of Verbena following benzylaminopurine application. Hort. Science 26(4): 391–392

    Google Scholar 

  • Webster AD (1980) Dwarfing rootstocks for plums and cherries. Acta Hort. 114:201–207

    Google Scholar 

  • Welander M (1985) In vitro shoot and root formation in apple cultivar Akero. Ann. Bot. 55: 249–261

    Google Scholar 

  • Ystaas Y & Froyness O (1996) Evaluation of size-controlling rootstocks for Stella and Ulster sweet cherries. Acta Hort. 410: 197–204

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ministry of Agriculture (SMAAR), Directorate of Agricultural Scientific Research, (DASR)-Douma, PO Box 113, Damascus, Syria

    AL-Sabbagh Muna, Abdul-Kader Ahmad & Kalhout Abdul-Rahman

  2. Agricultural Engineering Faculty, Horticulture Department, Alleppo University, Aleppo, Syria

    Khoder Mahmoud

Authors
  1. AL-Sabbagh Muna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abdul-Kader Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Khoder Mahmoud
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kalhout Abdul-Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Muna, AS., Ahmad, AK., Mahmoud, K. et al. In vitro propagation of a semi-dwarfing cherry rootstock. Plant Cell, Tissue and Organ Culture 59, 203–208 (1999). https://doi.org/10.1023/A:1006444925445

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006444925445

Search

Navigation