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Regeneration of Echinacea purpurea: Induction of root organogenesis from hypocotyl and cotyledon explants

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Abstract

An in vitro propagation system was developed for Echinacea purpureaL. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Root organogenesis from Echinacea purpurea hypocotyl explants was effectively induced by indolebutyric acid. Indoleacetic acid was found to be less effective than indolebutyric acid while treatments with naphthaleneacetic acid were ineffective for induction of root organogenesis. The results of this study have established a micropropagation system for Echinacea purpurea that will provide axenic plant material for further investigations into medicinally active biochemicals and the mass production of high-quality Echinacea purpurea root tissues for the commercial market.

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Authors and Affiliations

  1. Department of Plant Agriculture, Biotechnology Division, University of Guelph, Guelph, Ontario, Canada, N1G 2W1

    Kristen L. Choffe, Susan J. Murch, Praveen K. Saxena & Praveen K. Saxena

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  1. Kristen L. Choffe
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  2. Susan J. Murch
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  3. Praveen K. Saxena
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Correspondence to Praveen K. Saxena.

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Choffe, K.L., Murch, S.J. & Saxena, P.K. Regeneration of Echinacea purpurea: Induction of root organogenesis from hypocotyl and cotyledon explants. Plant Cell, Tissue and Organ Culture 62, 227–234 (2000). https://doi.org/10.1023/A:1006444821769

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  • DOI: https://doi.org/10.1023/A:1006444821769

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