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Transmission of organelle genomes in citrus somatic hybrids

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Abstract

Restriction fragment length polymorphisms (RFLPs), were used to analyze the organelle composition of two-year-old trees, recovered from two different experiments: protoplasts from embryogenic cell suspensions of `Succari' sweet orange (C. sinensis L. Osbeck) were fused with leaf protoplasts of Citropsis gilletiana Swingle & M. Kell or to leaf protoplasts of Atalantia ceylanica(Arn.) Oliv. The somatic hybrids of both fusion combinations had the mitochondrial genome from the embryogenic partner. In some somatic hybrids, non-parental fragments were observed among the mitochondrial patterns. Somatic hybrids between `Succari' + Atalantia had plastid DNA from the embryogenic parent, while the somatic hybrids of `Succari' + Citropsis all had both parental chloroplast genomes. The relative abundance of organelle DNAs in the donor embryogenic and leaf cells may explain the consistent transmission of the embryogenic parent mitochondrial DNA and the inheritance of the chloroplast genome from either parent.

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Authors and Affiliations

  1. Institute of Food and Agricultural Sciences, Horticultural Sciences Dept., University of Florida, P.O. Box 110690, USA, Gainesville, FL, 32611-0690, USA

    Cristina D. Moreira, Christine D. Chase & Jude W. Grosser

  2. Institute of Food and Agricultural Sciences, CREC, University of Florida, P.O. Box 1088, Lake Alfred, FL, 33850, USA

    Fred G. Gmitter Jr

Authors
  1. Cristina D. Moreira
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  2. Christine D. Chase
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  3. Fred G. Gmitter Jr
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  4. Jude W. Grosser
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Correspondence to Cristina D. Moreira.

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Moreira, C.D., Chase, C.D., Gmitter, F.G. et al. Transmission of organelle genomes in citrus somatic hybrids. Plant Cell, Tissue and Organ Culture 61, 165–168 (2000). https://doi.org/10.1023/A:1006434221970

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