Skip to main content
SpringerLink
Log in

Influence of genotype and gelling agents on in vitro regeneration by organogenesis in sunflower

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Fourteen recombinant inbred lines of sunflower (Helianthus annuus L.) and their parents (PAC-2 and RHA-266) were tested for their organogenesis ability. Seeds were surface sterilized and germinated on hormone free half strength MS basal medium containing 10 g l-1 sucrose solidified with five different gelling agents: Phytagar (Gibco laboratoires) 3 g l-1, Phytagel (Sigma) 3 g l-1, Agarose (Sigma) 5 g l-1, Arcagel (Sigma) 4 g l-1 and Agar-Agar (Fisher France) 7 g l-1. Cotyledons from 2-day-old seedlings were split in half and the four explants of each seed were cultived in 55 mm diameter petri dishes containing 10 ml of MS medium supplemented with 50 μM KNO3, 1 μM myo-inositol, 5 μM casein hydrolysate, 4.4 μM of BA and 5.4 μM of NAA solidified with the same gelling agents. The experimental design was a randomized complete block with 3 replications. A replicate for each genotype consisted of ten petri dishes containing four explants. The statistical analysis showed significant differences among genotypes and gelling agents. Of the fourteen recombinant inbred lines tested `C93' presented the highest values for all regeneration traits in the five different media and it was better than the best parent. Agarose and Agar-Agar were more better than other gelling agents for shoot induction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alibert G, Aslane-Chanabé CH & Burrus M (1994) Sunflower tissue and cell cultures and their use in biotechnology. Plant Cell. Biochem. 32: 31–44

    Google Scholar 

  • Beruto M, Beruto D & Debergh P (1999) Influence of agar on in vitro. cultures: I. Physicochemical properties of agar and agar gelled media in vitro. Cell. Dev. Biol. Plant 35: 86–93

    Google Scholar 

  • Bornam CH & Vogelman TC (1984) Effect of rigidity of gel medium on benzyladenine?induced adventitious bud formation and vitrification in vitro in Picea abies. Physiol. Plant. 61: 505–512

    Google Scholar 

  • Brand MH (1993) Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviveberry (Amelanchier arborea) shoots in vitro. Physiol. Plant. 61: 505–512

    Google Scholar 

  • Chraibi BKM, Castelle JC, Latche A, Roustan JP & Fallot J (1992) Enhancement of shoot regeneration potential by liquid medium culture from nature cotyledons of sunflower (Helianthus annuus L.) Plant Cell. Rep. 10: 617–620

    Google Scholar 

  • Debergh P, Harbaoui Y & Lemeur R (1981) Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special référence to water potential. Physiol Plant. 53: 181–187

    Article  Google Scholar 

  • Debergh PC (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol. Plant 59: 270–276

    Article  Google Scholar 

  • Debergh P, Aitken?Christie J, Cohen D, Grout B, Von Arnold S, Zimmerman R & Zib M (1992) Reconsideration of the term 'vitrification' as used in micropropagation. Plant Cell. Tiss. Org. Cult. 40: 135–140

    Google Scholar 

  • Deglene L, Lesignes P, Alibert G & Sarrafi A (1997), Genetic control of organogenesis in cotyledons of sunflower (Helianthus annuus L.). Plant Cell. Tiss. Org. Cult. 48: 127–130

    Article  Google Scholar 

  • Espinasse A, Lay C & Volin J (1989) Effects of growth regulator concentrations and explant size on shoot organogenesis from callus derived from zygotic embryos of sunflower (Helianthus annuus L.). Plant Cell. Tiss. Org. Cult. 17: 171–181

    Article  Google Scholar 

  • Ghaemi M & Sarrafi A (1993) Analysis of anther culture to measure genetic variability for embryogenesis in tetraploid wheat (Triticum turgidum var.). J. Genet. Breed. 47: 295–298

    Google Scholar 

  • Kusumoto M (1980) Effects of coconut milk, agar and sucrose concentrations, and media pH on the proliferation of Cymbidium protocorm?like bodies cultured in vitro. J. Jap. Soc. Hortic. Sci. 48: 503–509

    Google Scholar 

  • Lupi MC, Bennici A, Locci F & Gennai D (1987) Plantlet formation from callus and shoot tip culture of Helianthus annuus (L.) Plant Cell. Tiss. Org. Cult. 11: 47–55

    Article  Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497

    Article  Google Scholar 

  • Nairn JB, Furneaux RH & Stevenson TT (1995) Identification of an agar constituent responsible for hydric control in micropropagation of radiata pine. Plant Cell Tiss. Org. Cult. 43: 1–11

    Article  Google Scholar 

  • Pâques M & Boxus PH (1987) 'Vitrification': review of literature. Acta Hortic. 212: 155–166

    Google Scholar 

  • Pochet B, Scoman V, Mestdagh MM, Moreau B & Andre P (1991) Influence of agar gel properties on the in vitro micropropagation of different clones of Thuja plicata. Plant Cell. Rep 10: 406–409

    Article  Google Scholar 

  • Power CJ (1987) Organogenesis from Helianthus annuus inbreds and hybrids from the cotyledons of zygotic embryos An. J. Bot. 74: 497–503

    Google Scholar 

  • Pugliesi C, Megale P, Cecconi F & Baroncelli S (1993) Organogenesis and embryogenesis in Helianthus tuberosus _ Helianthus tuberosus. Plant Cell. Tiss. Org. Cult. 33: 187–193

    Article  Google Scholar 

  • Quimio CA & Zapata FJ (1990) Diallel analysis of callus induction and green?plant regeneration in rice anther culture. Crop Sci. 30: 188–192

    Article  Google Scholar 

  • Reeds DA (1969) Structure, conformation and mecanism in the germination of polysaccharide gels and networks. Adv. Carbohydrate chem. Biochem. 24: 267–332

    Google Scholar 

  • Sarrafi A, Bolandi AR, Serieys H, Berville A & Alibert G (1996a) Analysis of cotyledon culture to measure genetic variability for organogenesis parameters in sunflower (Helianthus annuus L.) Plant Science 121: 213–219

    Article  Google Scholar 

  • Sarrafi A, Roustan JP, Fallot J & Alibert G (1996b) Genetic analysis of organogenesis in the cotyledons of zygotic embryos of sunflower (Helianthus annuus L.). Theor. Appl. Genet. 92: 225–229

    Google Scholar 

  • Singhas (1984) Influence of two commercial agars on in vitro shoot proliferation of 'Almey' Crabapple and 'Seckel' Pear. Hort. Sci. 19: 227–228

    Google Scholar 

  • Wirtzens B, Scowcroft WR, Downes RW & Larkin PJ (1988) Tissue culture and plant regeneration from sunflower (Helianthus annuus) and interspecific hybrids (H. tuberosus _ H. annuus). Plant. Cell. Tiss. Org. Cult. 13: 61–76

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 939 INRA, 18 chemin de Borde Rouge, B.P. 107, F-31326, Castanet-Cedex, France

    E. Flores Berrios, L. Gentzbittel, G. Alibert & A. Sarrafi

  2. INRA, URGAP Grandes Cultures, 2 place Pierre Viala, F-34060, Montpellier, France (

    H. Serieys

Authors
  1. E. Flores Berrios
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Gentzbittel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Serieys
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Alibert
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Sarrafi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berrios, E.F., Gentzbittel, L., Serieys, H. et al. Influence of genotype and gelling agents on in vitro regeneration by organogenesis in sunflower. Plant Cell, Tissue and Organ Culture 59, 65–69 (1999). https://doi.org/10.1023/A:1006433607812

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006433607812

Search

Navigation