Skip to main content
SpringerLink
Log in

Effect of genotype and season on gynogenesis efficiency in Gerbera

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The effects of season, genotype and their interaction on haploid production were evaluated on four genotypes responsive to gynogenesis. Naked mature unfertilised ovules, collected from April to October, were cultivated on modified MS basal medium plus 0.88 μM 6-benzyladenine and 0.57 μM indol-3-acetic acid. After 30–60 days, recovered calli were transferred to a MS basal medium with 8.8 μM BA and 0.57 μM IAA for regeneration. Genotype and season of ovule collection substantially interact in the recovery of haploid calli. Between the four genotypes analysed, two gave more calli in the spring, one in the autumn and the fourth showed only weak differences between seasons. Shoot recovery depended upon both the season of ovule collection and the genotype but no significant interaction was shown by our data. The ability to produce haploid callus is not predictive for efficient shoot regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahmim M & Vieth J (1986) Production de plantes haploides de Gerbera jamesonii par culture in vitro d'ovules. Can. J. Bot. 64: 2355–2357

    Article  Google Scholar 

  • Cappadocia M, Chrétien L & Laublin G (1987) Production of haploids in Gerbera jamesonii via ovule culture: influence of fall versus spring sampling on callus formation and shoot regeneration. Can. J. Bot. 66: 1107–1110

    Google Scholar 

  • Compton ME (1994) Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tiss. Org. Cult. 37: 217–242

    Google Scholar 

  • Miyoshi K & Asakura N (1996) Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii). Plant Cell Rep. 16: 1–5

    Article  Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Article  Google Scholar 

  • Tosca A, Lombardi M, Marinoni L, Conti L & Frangi P (1990) Genotype response to in vitro gynogenesis technique in Gerbera jamesonii. Acta Hort. 280: 337–340

    Google Scholar 

  • Tosca A, Pandolfi R, Citterio S, Fasoli A & Sgorbati S (1995) Determination by flow cytometry of the chromosome doubling capacity of colchicine and oryzalin in gynogenetic haploids of Gerbera. Plant Cell Rep. 14: 455–458

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fondazione Minoprio, Viale Raimondi 54, 22070, Como, Italy requests for offprints)

    Alberto Tosca, Licia Arcara & Piero Frangi

Authors
  1. Alberto Tosca
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Licia Arcara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Piero Frangi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tosca, A., Arcara, L. & Frangi, P. Effect of genotype and season on gynogenesis efficiency in Gerbera. Plant Cell, Tissue and Organ Culture 59, 77–80 (1999). https://doi.org/10.1023/A:1006418619992

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006418619992

Search

Navigation