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Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro

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Abstract

Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to determine the optimum environment for in vitro growth and ex vitro survival. Plantlet growth increased with an increasing number of air exchanges of the culture vessel, exhibiting greatest shoot weight, total fresh weight, leaf area, and chlorophyll content at 4.4 h−1 of air exchanges. High sucrose concentration (30 g l−1) increased root weight but reduced shoot growth. Net photosynthetic rates of the plantlets were greatest when sucrose was not added to the medium. On the other hand, ex vitro survival of the plantlets was not influenced by sucrose concentration. In the experiment on difference in photoperiod and dark period temperatures (DIF) and photosynthetic photon flux (PPF), plantlet growth increased as DIF and PPF levels increased. Particularly, increasing PPF level had a more distinctive effect on plantlet growth than increasing DIF level. The interaction of DIF × PPF was also significant, showing the greatest plantlet growth in positive DIF (+8 DIF) and a high PPF (210 μmol m−2 s−1). In conclusion, the results of this experiment suggest that increased number of air exchanges of the culture vessel, decreased sucrose concentration, and positive DIF in combination with high PPF level enhanced growth and acclimatization of Rehmannia glutinosa plantlets.

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, 361-763, Korea

    Yong-Yi Cui, Eun-Joo Hahn, Kee-Yoeup Paek & Kee-Yoeup Paek

  2. Faculty of Horticulture, Chiba University, Matsudo, Chiba, 271-8510, Japan

    Toyoki Kozai

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  1. Yong-Yi Cui
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  2. Eun-Joo Hahn
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  3. Toyoki Kozai
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  4. Kee-Yoeup Paek
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Correspondence to Kee-Yoeup Paek.

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Cui, YY., Hahn, EJ., Kozai, T. et al. Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro . Plant Cell, Tissue and Organ Culture 62, 219–226 (2000). https://doi.org/10.1023/A:1006412321864

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