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Plant regeneration from protoplasts of a commercial Asian long-grain javanica rice

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Abstract

A reproducible plant regeneration system has been developed for protoplasts from embryogenic cell suspension cultures of the commercial Asian long-grain javanica rice, Oryza sativa cv. Azucena. Protoplasts were isolated routinely from cell suspensions with yields of 5.5–12.0 × 106 g-1 fresh weight. A membrane filter nurse-culture method was adopted and was essential to support sustained mitotic division of protoplast-derived cells, leading to cell colony formation. The protoplast plating efficiency was higher when suspension cells of Lolium multiflorum, rather than those of the japonica rice O. sativa L. cv. Taipei 309, were employed as nurse cells. A two-step shoot regeneration procedure, in which protoplast-derived calli were cultured initially on medium semi-solidified with 1% (w/v) agarose followed by culture on medium containing 0.4% (w/v) agarose, induced plant regeneration from protoplast-derived calli. Fifteen percent of protoplast-derived tissues regenerated shoots; tissues not subjected to this treatment failed to develop shoots.

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Authors and Affiliations

  1. Institute of Genetics, Fudan University, Shanghai, 200433, People's Republic of China

    K. Tang & X. Sun

  2. Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK

    D. An, J.B. Power, E.C. Cocking & M.R. Davey

Authors
  1. K. Tang
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  2. X. Sun
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  3. D. An
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  4. J.B. Power
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  5. E.C. Cocking
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  6. M.R. Davey
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Corresponding author

Correspondence to M.R. Davey.

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Tang, K., Sun, X., An, D. et al. Plant regeneration from protoplasts of a commercial Asian long-grain javanica rice. Plant Cell, Tissue and Organ Culture 60, 79–82 (2000). https://doi.org/10.1023/A:1006408109595

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