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Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

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Abstract

X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing. were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%. respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.

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  1. National key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China

    Jihong Liu & Xiuxin Deng

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  1. Jihong Liu
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  2. Xiuxin Deng
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Liu, J., Deng, X. Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis. Plant Cell, Tissue and Organ Culture 59, 81–87 (1999). https://doi.org/10.1023/A:1006399302843

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