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Improved embryogenesis from anther culture and plant regeneration in timothy

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Abstract

Timothy (Phleum pratense L.) is an important forage grass grown in northern temperate areas. Development of haploid cell culture techniques for timothy has been limited due to the recalcitrance of timothy in tissue culture. In this study, timothy anther culture techniques were established. Liquid PG-96 (Pulli and Guo, 1996) induction medium significantly promoted embryo yield; the best result was 800–1000 embryos (calli) per 100 anthers. Genotype was an important factor in androgenetic embryogenesis of timothy. Embryos were obtained from 16 genotypes out of the 28 genotypes tested. The optimum stage for microspore development was between the very late uninucleate stage and the binucleate stage. Cold pretreatment applied to the donor plants (spikes) increased embryo yield. Despite a high embryo induction rate, green plant regeneration rate was relatively low. The frequency of albinos was reduced by use of low light intensity conditions during regeneration. Over 300 green plants were recovered.

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Authors and Affiliations

  1. Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, 20014, Turku, Finland

    Yang-Dong Guo, Pirjo Sewón & Seppo Pulli

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  1. Yang-Dong Guo
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  2. Pirjo Sewón
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  3. Seppo Pulli
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Guo, YD., Sewón, P. & Pulli, S. Improved embryogenesis from anther culture and plant regeneration in timothy. Plant Cell, Tissue and Organ Culture 57, 85–93 (1999). https://doi.org/10.1023/A:1006392411276

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