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Molecular Genetic Studies of Chromosome 11 And Chromosome 22q DNA Sequences in Pediatric Medulloblastomas

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Abstract

Medulloblastomas are primitive neuroectodermal tumors (PNETs) of the cerebellum with poorly understood pathogenesis. Previous molecular studies suggested a role for loci on chromosome 11 in the development of medulloblastomas-PNETs. In order to identify the frequency of loss and eventually the extent of allelic loss on chromosome 11, we have examined 23 pediatric medulloblastomas for loss of heterozygosity (LOH) with 16 polymorphic microsatellites. Our data reveal that LOH on 11p or 11q occurs rarely (13%) suggesting the unlikely involvement of chromosome 11 in most cases of medulloblastomas. The same frequency of LOH in medulloblastomas was detected using 8 microsatellites on 22q. Alterations of microsatellite length were found in only 4/594 PCR analyses using 28 markers located on chromosomes 2, 9, 11, 18, and 22, demonstrating that genomic instability is uncommon in medulloblastomas.

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Authors and Affiliations

  1. Unité 383 INSERM Clinique Lamy, Paris, France

    Servane Lescop & Corinne Besnard-Guerin

  2. Laboratory of Neuropathology, Hôpital Necker–Enfants Malades, Paris, France

    Arielle Lellouch-Tubiana

  3. Department of Pediatrics, Institut Gustave-Roussy, Villejuif, France

    Gilles Vassal

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  1. Servane Lescop
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  2. Arielle Lellouch-Tubiana
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  3. Gilles Vassal
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  4. Corinne Besnard-Guerin
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Lescop, S., Lellouch-Tubiana, A., Vassal, G. et al. Molecular Genetic Studies of Chromosome 11 And Chromosome 22q DNA Sequences in Pediatric Medulloblastomas. J Neurooncol 44, 119–127 (1999). https://doi.org/10.1023/A:1006387518100

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