Skip to main content

Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants

Abstract

The hypothesis that physiologically activeconcentrations of salicylic acid (SA) and itsderivatives can confer stress tolerance in plants wasevaluated using bean (Phaseolus vulgaris L.) andtomato (Lycopersicon esculentum L.). Plantsgrown from seeds imbibed in aqueous solutions (0.1--0.5 mM) of salicylic acid or acetyl salicylic acid(ASA) displayed enhanced tolerance to heat, chillingand drought stresses. Seedlings acquired similarstress tolerance when SA or ASA treatments wereapplied as soil drenches. The fact that seedimbibition with SA or ASA confers stress tolerance inplants is more consistent with a signaling role ofthese molecules, leading to the expression oftolerance rather than a direct effect. Induction ofmultiple stress tolerance in plants by exogenousapplication of SA and its derivatives may have asignificant practical application in agriculture,horticulture and forestry.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Benson EE (1990) Free radical damage in stored plant germplasm. International board for plant genetic resources. Rome.

  2. 2.

    Bi Y-M, Kenton P, Murr L, Darby R and Draper J (1995) H2O2 does not function downstream of salicylic acid in the induction of PR protein expression. Plant J 8: 235-245

    Google Scholar 

  3. 3.

    Chen Z, Silva H and Klesig RF (1993) Active oxygen species in the induction of plant systemic acquired resistance by SA. Science 262: 1883-1886

    Google Scholar 

  4. 4.

    Cheng IF, Zhao CP, Amolins A, Galazka M and Doneski L (1996) A hypothesis for the in vivo antioxidant activity of salicylic acid. Biometals 9: 285-290

    Google Scholar 

  5. 5.

    Conrath U, Chen Z, Rizigliano JR and Klessig DF (1995) Two inducers of plant defence responses, 2, 6-dichloroisonicotinic acid and salicylic acid inhibit catalase activity in tobacco. USA: Proc Natl. Acad. Sci. 92: 7143-7147

    Google Scholar 

  6. 6.

    Dat JF, Foyer CH and Scott IM (1998) Changes in salicylic acid and antioxidants during induced thermotolerance in mustard seedlings. Plant Physiol 118: 1455-1461

    Google Scholar 

  7. 7.

    Dat JF, Lopez-Delgado H, Foyer CH and Scott IM (1998) Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid and heat acclimation of mustard seedlings. Plant Physiol 116: 1351-1357

    Google Scholar 

  8. 8.

    Enyedi AJ, Yalpani N, Silverman P and Raskin I (1992) Signal molecules in systemic plant resistance to pathogens and pests. Cell 70: 879-886

    Google Scholar 

  9. 9.

    Fletcher RA and Hofstra G (1988) Triazoles as potential plant protectants. In: Berg D and Plempel M (eds) Sterol Biosynthesis Inhibitors: Pharmaceutical and Agricultural Aspects. Cambridge: Ellis Horwood Ltd., pp. 321-331

    Google Scholar 

  10. 10.

    Keppler LD and Novasky A (1987) The initiation of membrane lipid peroxidation during bacteria-induced hypersensitive reaction. Physiol. Molec. Plant Path. 30: 233-245

    Google Scholar 

  11. 11.

    Klessig DF and Malamy J (1994) The salicylic acid signal in plants. Plant Molec. Biol. 26: 1439-1458

    Google Scholar 

  12. 12.

    Kraus TE and Fletcher RA (1994) Paclobutrazol protects wheat seedlings from heat and paraquat injury. Is detoxification of active oxygen species involved? Plant Cell Physiol. 35: 45-52

    Google Scholar 

  13. 13.

    Levin A, Teneken R, Dixon RA and Lamb CJ (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79: 583-593

    Google Scholar 

  14. 14.

    Lopez-Delgado H, Dat JF, Foyer CH and Scott IM (1998) Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2. J Exp Bot 49: 713-720

    Google Scholar 

  15. 15.

    McKersie BD, Senaratna T, Walker MA, Kendall EJ and Hetherington PR (1988) Deterioration of membranes during aging 161 in plants: Evidence for free radical mediation. In: Nooden LD and Leopold AC (eds) Senescence and aging in plants. London: Academic press, pp. 442-464

    Google Scholar 

  16. 16.

    Mehdy MC (1994) Active oxygen species in plant defence against pathogens. Plant Physiol. 105: 467-472

    Google Scholar 

  17. 17.

    Mur LAJ, Naylor G, Warner SAJ, Sugars JM, White RF and Draper J (1996) Salicylic acid potentiates defence gene expression in leaf tissues exhibiting acquired resistance to pathogen attack. Plant J. 9: 559-571

    Google Scholar 

  18. 18.

    Neuenschwander U, Vernooij B, Friedrich L, Uknes S, Kessmann H and Ryals J (1995) Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance. Plant J. 8: 227-233

    Google Scholar 

  19. 19.

    Raskin I (1992) Role of salicylic acid in plants. Ann. Rev. Plant Physiol. Molec. Biol. 43: 439-463

    Google Scholar 

  20. 20.

    Ryals JA, Neuenschwander UH, Willits mg, Molina A, Steiner HY and Hunt MD (1996) Systemic acquired resistance. Plant Cell 8: 1809-1819

    Google Scholar 

  21. 21.

    Senaratna T, McKay CE, McKersie BD and Fletcher RA (1988) Uniconazole induced chilling tolerance in tomato and its relationship to antioxidant content. J. Plant Physiol. 133: 56-61

    Google Scholar 

  22. 22.

    Senaratna T, McKersie BD and Borochov A (1987) Desiccation and free radical mediated changes in plant membranes. J. Exp. Bot. 38: 2005-2014

    Google Scholar 

  23. 23.

    Senaratna T, McKersie BD and Stinson RH (1985) Simulation of dehydration injury by free radicals. Plant Physiol. 77: 472-474

    Google Scholar 

  24. 24.

    Senaratna T, McKersie BD and Stinson RH (1985) Antioxidant levels in germinating soybean seed axes in relation to free radical and dehydration tolerance. Plant Physiol. 78: 168-171

    Google Scholar 

  25. 25.

    Shirasu K, Nakajima H, Rajasekar VK, Dixon RA and Lamb C (1997) Salicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signal in the activation of defence mechanisms. The Plant Cell 9: 261-270

    Google Scholar 

  26. 26.

    Srivastava MK and Dwivedi UN (1998) Salicylic acid modulates glutathione metabolism in pea seedlings. J. Plant Physiol. 153: 404-409

    Google Scholar 

  27. 27.

    Sun WQ, Irving TC and Leopold AC (1994) The role of sugar, vitrification and membrane phase transition in seed desiccation tolerance. Physiol. Plant. 90: 621-628

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Tissa Senaratna.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Senaratna, T., Touchell, D., Bunn, E. et al. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation 30, 157–161 (2000). https://doi.org/10.1023/A:1006386800974

Download citation

  • stress tolerance
  • acetyl salicylic acid
  • salicylic acid
  • heat
  • drought
  • cold