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Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon

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Abstract

The influence of light incubation during embryo germination on shoot organogenesis from cotyledons of four diploid watermelon [Citrullus lanatus (Thumb.) Matsum. & Nakai cultivars was examined. Germinating embryos in darkness significantly improved the number of explants that produced harvestable shoots during the 6 week incubation period on shoot regeneration medium under a 16-h photoperiod. The percentage of explants with shoots more than doubled for `Crimson Sweet' and was about 1.5-fold greater for `Sweet Gem' and `Yellow Doll' when embryos were germinated in darkness. The percentage of explants with shoots was not significantly improved for `Minilee' by pretreating seedlings in darkness. This study demonstrates that optimal shoot regeneration can be obtained by germinating embryos in darkness before preparing cotyledon explants.

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Authors and Affiliations

  1. School of Agriculture, University of Wisconsin-Platteville, 1 University Plaza, Platteville, WI, 53818

    Michael E. Compton

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  1. Michael E. Compton
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Compton, M.E. Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon. Plant Cell, Tissue and Organ Culture 58, 185–188 (1999). https://doi.org/10.1023/A:1006364013126

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