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Effect of low doses of gamma irradiation on in vitro growth of grapevine

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Abstract

Shoot tips and single node explants of two rootstocks (R.99 and 3309) and two varieties (‘Helwani’ and ‘Cabernet Franc’) of grapevine cultured on DSD1 media for a period of 60 days, were irradiated with 0, 2, 5 and 7 Gy doses of gamma irradiation. Shoot length of ‘Helwani’ and ‘Cabernet Franc’ was increased by 7 Gy irradiation. The 5 Gy dose increased the number of roots in plants of the two rootstocks and ‘Helwani’. Root length of ‘Helwani’ and ‘Cabernet Franc’ at the 2 and 7 Gy doses were significantly higher than those of the non-irradiated control. A similar effect was noticed on R.99 rootstock subjected to 5 Gy. Five Gy also increased the dry weight of the R.99 rootstock, whereas 2 and 7 Gy had a similar effect on ‘Helwani’ and ‘Cabernet Franc’. Number of leaves of plants exposed to 5 and 7 Gy was increased when compared with the non-irradiated control.

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References

  • Abacus Concepts (1994) StatView, version 4. 02. Abacus Concepts. Berkeley, CA

  • Al-Bashir M (1995) The effect of low doses of gamma radiation on root formation of olive cuttings. Agrobiol. Res. 48: 179–183

    Google Scholar 

  • Alleweldt G & Possingham JV (1988) Progress in grapevine breeding. Theor. Appl. Genet. 75: 669–673

    Article  Google Scholar 

  • Al-Oudat M (1990) Effect of low dose gamma irradiation on onion yield. Ann. Biol. 6: 61–67

    Google Scholar 

  • Al-Safadi B & Simon PW (1996) Gamma irradiation induced variation in carrots. J. Amer. Soc. Hort. Sci. 121: 599–603

    Google Scholar 

  • Al-Safadi B & Simon PW (1990) The effects of gamma irradiation on the growth and cytology of carrot ( Dacus carota L.) Tissue Culture. Environ. Expt. Bot. 30: 361–371

    Article  Google Scholar 

  • Antonov M(1985) Effect of gamma irradiation and storage duration of maize seeds on certain biochemical changes in the grain, pasteniev. D. Nauki. 19–24

  • Antonov M, Velichov P, Tsonev TS & Angelov M (1989) Effect of gamma and laser irradiation on maize seeds and plants. ESNA, XXth Annual Meeting, Wageningen, the Netherlands (p. 44).

  • Bajaj YPS (1970) Effect of gamma irradiation on growth, RNA, protein and nitrogen contents of bean callus cultures. Ann. Bot. 34: 1089–1096

    CAS  Google Scholar 

  • Baker AM, Ashour NE, Elbasyouni SZ & Saed AM (1976) Response of the photosynthetic apparatus of corn (Zea mays) to presowing seed treatment with gamma rays and ammonium molybdate. Environ. Exp. Bot. 16: 217–222

    Article  Google Scholar 

  • Bouquet A (1989) Interet des téchniques de culture in vitro pour l'amélioration génétique de la vigne, Bull. O.I.V., 697–698: 179-192

    Google Scholar 

  • Berezina NM & Kaushanskii DA (1989) Presowing irradiation of plant seeds, Oxonian Press PVT. Ltd., New Delhi

  • Fowler DB & MacQueen KF (1972) Effect of low doses of gamma radiation on yield and other agronomic characters of spring wheat (Triticum asetivum ) Radiat. Bot. 12: 349–353

    Google Scholar 

  • Freidman ST (1985) Pre-sowing radiation stimulation of crop seeds. ESNA Newsletter 126–148. Warszawa

  • Galzy R (1985) Les possibilite de conservation in vitro d'une collection de clones de vignes. Bull. O.I.V., V. 650: 378–390

    Google Scholar 

  • Ghiorghita AGI, Toth ET & Popescu TT (1985) The influence of physiological stage of seeds at the irradiation moment on the effects induced by small doses of gamma rays 60Co in Triticum aestivum. Rev. Roum. Biol. Ser. Biol. Veg. 30: 151–158

    CAS  Google Scholar 

  • Gray DJ & Meredith CP (1992) Grape biotechnology. In: Hammerschlage FA, Litz RE (eds) Biotechnology of Perennial Fruit Crops, Chap. 9 (pp 229–261). C.A.B. International, Wallingford, UK

  • Grossman HH & Craig R (1982) The effect of gamma irradiation of seeds on germination and plant morphology of Pelargonium hortorum L.H. Bailey. J. Amer. Soc. Hort. Sci. 107: 72–75

  • Kuksova VB, Piven NM & Gleba YY (1997) Somaclonal variation and in vitro induced mutagenesis in grapevine. Plant Cell Tiss. Org. Cult. 49: 17–27

    Article  CAS  Google Scholar 

  • Lage LSC & Esquibel MA (1997) Growth stimulation produced by methylene blue treatment in sweet potato. Plant Cell Tiss. Org. Cult. 48: 77–81

    Article  CAS  Google Scholar 

  • Lebrun L, Rajasekaran K & Mullins MG (1985) Selection in vitro for NaCl tolerance in Vitis rupestris Scheele. Ann. Bot., V. 56: 733–739

    Google Scholar 

  • Lima DaSilva A (1995) Mutagenese in vitro de porte-greffes de la vigne: Methodlogie d'irradiation, d'isolement et caracteresation de mutants. These DOC. Uni. Bordeaux II. No 375: 135

    Google Scholar 

  • Lima DaSilva A & Doazan JP (1995) Une methode d'irradiation aux rayon gamma appliquée à des porte-greffes de vigne in vitro. J. Int. Sci. Vigne Vin V.29: 1–9

    Google Scholar 

  • Maghraby GM (1987) Effect of radiation and some micronutrients on some legumes. Thesis submitted in partial fulfilment of the requirements for the degree of master of science. Zagazig University (Banha Branch)

  • Reustle G & Alleweldt G (1990) Isolation and culture of grapevine protoplasts. In: Proc. V Int. Symp. Grape Breeding Vitis Special: 423–431

  • Sheppard SC & Evenden WG (1986) Factors controlling the response of field crops to very low doses of gamma irradiation of seed. Can. J. Plant Sci. 66: 431–442

    Article  Google Scholar 

  • Spiegel-Roy P & Koghba J (1973) Radiosensitivity of shamouti orange (Citrus sinensis ) seeds and buds. Rad. Bot. 13: 105–110

    Article  Google Scholar 

  • Torregrosa L & Bouquet A (1993) Culture in vitro: Apports actuels. et perspectives pour la multiplication et l'amelioration de la vigne. Prog. Agr-vit., V 5–6: 113-114

    Google Scholar 

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Charbaji, T., Nabulsi, I. Effect of low doses of gamma irradiation on in vitro growth of grapevine. Plant Cell, Tissue and Organ Culture 57, 129–132 (1999). https://doi.org/10.1023/A:1006360513965

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  • DOI: https://doi.org/10.1023/A:1006360513965

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