Abstract
When thin cell layers (TCLn), isolated from the nodalregion of the hypocotyl of 5 day old seedlings, werecultured in medium M1 with IAA and BA, entire plantswere obtained from L. mutabilis explants andprolific shoots from L. albus explants.Histological studies carried out on L. mutabilisTCLn showed that meristematic cells, present in theexplants at the time of inoculation, gave rise to theformation of a primary meristem resulting inorganogenesis. In TCLn where apical or axillarymeristematic cells were absent, we observed theformation of a nodular structure with a cambium-likelayer at its periphery which divided to form a primarymeristem leading to organogenesis. Plant regenerationfrom TCLn may be useful in genetic transformationassays.
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Mulin, M., Bellio-Spataru, A. Organogenesis from hypocotyl thin cell layers of Lupinus mutabilis and Lupinus albus. Plant Growth Regulation 30, 177–183 (2000). https://doi.org/10.1023/A:1006345401325
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DOI: https://doi.org/10.1023/A:1006345401325