Skip to main content
SpringerLink
Log in

Effect of NaCl stress on H2O2 metabolism in rice leaves

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The effect of NaCl stress on H2O2 metabolismin detached rice leaves was studied. NaCl (200 mM)treatment did not cause the accumulation ofH2O2 and resulted in no increase in lipidperoxidation and membrane leakage of leaf tissues. The activities of peroxidase, ascorbate peroxidase,superoxide dismutase, and glutathione reductase wereobserved to be greater in NaCl-stressed rice leavesthan in control leaves. However, glycolate oxidasewas lower in NaCl-treated rice leaves than in thecontrol leaves. There was no difference in catalaseactivity between NaCl and control treatments. Theseresults suggest that some antioxidant enzymes can beactivated in response to oxidative stress induced byNaCl.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Aono M, Kubo A, Saji H, Natori T, Tanaka K and Kondo N (1991) Resistance to active oxygen toxicity of transgenic Nicotiana tabacum that expresses the gene for glutathione reductase from Escherichia coli. Plant Cell Physiol 32: 691-697

    Google Scholar 

  2. Asada K (1992) Ascorbate peroxidase-a hydrogen peroxide scavenging enzyme in plants. Physiol Plant 85: 235-241

    Google Scholar 

  3. Asada K and Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DC and Arntzen CJ (eds) Photoinhibition, pp 227-287. Amsterdam: Elsevier

    Google Scholar 

  4. Badiani M, Schenone G, Paolacci AR and Fumagalli I (1993) Daily fluctuations of antioxidants in bean (Phaseolus vulgaris L.) leaves as affected by the presence of ambient pollutants. Plant Cell Physiol 34: 271-279

    Google Scholar 

  5. Bowler C, Slooten L, Vandonbranden S, De Rycke R, Booterman J, Sysbesma C, Van Montagu M and Inze D (1991) Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J 10: 1723-1732

    Google Scholar 

  6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254

    Google Scholar 

  7. Brennan T, Rychter A and Frenkel C (1979) Activity of enzymes involved in the turnover of hydrogen peroxide during senescence. Bot Gaz 140: 384-388

    Google Scholar 

  8. Del Rio L, Sandalio LM, Palma JM, Bueno P and Corpus FJ (1992) Metabolism of oxygen radicals in peroxisomes and cellular implication. Free Rad Biol Med 13: 557-580

    Google Scholar 

  9. Foster JG, and Hess JL (1980) Superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66: 482-487

    Google Scholar 

  10. Gossett DR, Millhollon EP and Lucas C (1994) Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci 34: 707-714

    Google Scholar 

  11. Gossett DR, Milhollon EP, Lucas C, Banks SW and Marney MM (1994) The effect of NaCl on antioxidant enzyme activities in callus tissue of salt-tolerant and salt-sensitive cultivars of cotton. Plant Cell Rep 13: 498-503

    Google Scholar 

  12. Gossett DR, Banks SW, Millhollon EP and Lucas C (1996) Antioxidant response to NaCl stress in a control and a NaCltolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine, and exogenous glutathione. Plant Physiol 112: 803-809

    Google Scholar 

  13. Halliwell B (1987) Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts. Chem Phys Lipids 44: 327-340

    Google Scholar 

  14. Heath RL and Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189-198

    Google Scholar 

  15. Hernandez JA, Corpas FJ, Gomez M, del Rio LA and Secilla F (1993) Salt-induced oxidative stress mediated by activated oxygen species in pea leaf mitochondria. Physiol Plant 89: 103-110

    Google Scholar 

  16. Herrandez JA, Olmos E, Corpas FJ, Sevilla F and del Rio LA (1995) Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci 105: 151-167

    Google Scholar 

  17. Inze U and Van Montagu M (1995) Oxidative stress in plants. Current Opinion Biotechnol 6: 153-158

    Google Scholar 

  18. Iturbe-Ormaetxe Y, Moran JF, Arrese-Igor C, Gogorcena Y, Klucas RV and Becana M (1995) Activated oxygen and antioxidant defences in iron-deficient pea plants. Plant Cell Environ 18: 421-429

    Google Scholar 

  19. Jana S and Choudhuri MA (1981) Glycolate metabolism of three submerged aquatic angiosperm during aging. Aquat Bot 12: 345-354

    Google Scholar 

  20. Kao CH (1980) Senescence of rice leaves IV. Influence of benzyladenine on chlorophyll degradation. Plant Cell Physiol 21: 1255-1262

    Google Scholar 

  21. Kato M and Shimizu S (1987) Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescening tobacco leaves: phenolic-dependent peroxidative degradation. Can J Bot 65: 729-735

    Google Scholar 

  22. MacAdam JW, Nelson CI, Sharp RE (1992) Peroxidase activity in the leaf elongation zone of tall fescue. I. Spatial distribution of ionically bound peroxidase activity in genotypes diferring in length of the elongation zone. Plant Physiol 99: 872-878

    Google Scholar 

  23. Mahan JR (1994) Thermal dependence of glutathione reductase: Thermal limitations on antioxidant protection in plants. Crop Sci 34: 1550-1556

    Google Scholar 

  24. Mukherjee SP and Choudhuri MA (1983) Implication of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58: 166-170

    Google Scholar 

  25. Nakano Y and Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867-880

    Google Scholar 

  26. Olmos E, Hernandez JA, sevilla F and Hellin E (1994) Induction of several antioxidant enzymes in the selection of a salt-tolerant cell line of Pisum sativum. J Plant Physiol 144: 594-598

    Google Scholar 

  27. Paoletti F, Aldinucci D, Mocali A and Capparini A (1986) A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem 154: 536-541

    Google Scholar 

  28. Perl A, Perl-Treves R, Galili S, Ariv D, Shalgi E, Malkin S and Galan E (1993) Enhanced oxidative-stress defence in transgenic potato expressing Cu, Zn superoxide dismutase. Theor Appl Genet 85: 568-576

    Google Scholar 

  29. Prasad TK, Anderson MD, Martin BA and Stewart CR (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6: 65-74

    Google Scholar 

  30. Sen Gupta A, Webb RP, Holaday AS and Allen RD (1993) Overexpression of superoxide dismutase protects plants from oxidative stress. Induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants. Plant Physiol 103: 1067-1073

    Google Scholar 

  31. Shalata A and Tal M (1998) The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol Plant 104: 167-174

    Google Scholar 

  32. Singha S and Choudhuri MA (1990) Effect of salinity (NaCl) stress on H2O2 metabolism in Vigna and Oryza seedlings. Biochem Physiol Pflanzen 186: 69-74

    Google Scholar 

  33. Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and dessication. New Phytol 125: 27-58

    Google Scholar 

  34. Smith IK, Vierheller TL and Thorne C (1989) Properties and functions of glutathione reductase in plants. Physiol Plant 77: 449-456

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Chuan Chi Lin

  2. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Ching Huei Kao

Authors
  1. Chuan Chi Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ching Huei Kao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, C.C., Kao, C.H. Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regulation 30, 151–155 (2000). https://doi.org/10.1023/A:1006345126589

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006345126589

Search

Navigation