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Establishment of callus and cell suspension cultures of Opuntia ficus-indica


Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 μM 6-furfurylaminopurine, 2.3 μM 2,4-dichlorophenoxyacetic acid, 1.0 μM 4-amino 3,5,6-trichloropicolinic acid, 400 mg l-1 casein hydrolysate and 3% sucrose. The same medium without agar was used for establishing cell suspensions.

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Llamoca-Zárate, R.M., Studart-Guimarães, C., Landsmann, J. et al. Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell, Tissue and Organ Culture 58, 155–157 (1999).

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  • Cactaceae
  • forage crops
  • in vitro propagation