Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 μM 6-furfurylaminopurine, 2.3 μM 2,4-dichlorophenoxyacetic acid, 1.0 μM 4-amino 3,5,6-trichloropicolinic acid, 400 mg l-1 casein hydrolysate and 3% sucrose. The same medium without agar was used for establishing cell suspensions.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Felker P (1995) Forage and fodder production and utilization. In: Barbera G, Inglese P, Pimiente-Barrios E & Arias-Jiménez E (eds) Agro-ecology, Cultivation and Uses of Cactus Pear, Paper 132 (pp. 144–154). FAO Plant Production and Protection Division, Roma
Fay MF & Gratton J (1992) Tissue culture of cacti and other succulents: a literature review and a report on micropropagation at Kew. Bradleya 10: 33–48
Gaff DF & Okong'o-Ogola O (1971) The use of non-permeating pigments for testing the survival of cells. J. Exp. Bot. 22: 756–758
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Palomino G, Dolezel J, Cid R, Brunner I, Mendez I & Rubluo A (1999) Nuclear genome stability of Mamillaria san-angelensis (Cactaceae) regenerants induced by auxins in long-term in vitro culture. Plant Science 141: 191–200
Stepan-Sarkissian G & Grey D (1990) Growth determination and medium analysis. In: Pollard JW & Walker JM (eds) Methods in Molecular Biology, Plant Cell and Tissue Culture, Vol. 6(pp. 13–27). The Humana Press, Clifton, New Jersey
About this article
Cite this article
Llamoca-Zárate, R.M., Studart-Guimarães, C., Landsmann, J. et al. Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell, Tissue and Organ Culture 58, 155–157 (1999). https://doi.org/10.1023/A:1006315729266
- forage crops
- in vitro propagation