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Establishment of callus and cell suspension cultures of Opuntia ficus-indica

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Abstract

Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed on Murashige & Skoog medium supplemented with 0.9 μM 6-furfurylaminopurine, 2.3 μM 2,4-dichlorophenoxyacetic acid, 1.0 μM 4-amino 3,5,6-trichloropicolinic acid, 400 mg l-1 casein hydrolysate and 3% sucrose. The same medium without agar was used for establishing cell suspensions.

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Authors and Affiliations

  1. Institute for Plant Virology, Microbiology and Biosafety, Federal Biological Research Centre for Agriculture and Forestry, Messeweg 11/12, D-3810, Braunschweig, Germany

    Rómulo M. Llamoca-Zárate & Joerg Landsmann

  2. Department of Biochemistry and Molecular Biology, Federal University of Ceará, P.O. Box 1065, 60001-970, Fortaleza, CE, Brazil

    Rómulo M. Llamoca-Zárate, Claúdia Studart-Guimarães & Francisco A.P. Campos

Authors
  1. Rómulo M. Llamoca-Zárate
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  2. Claúdia Studart-Guimarães
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  3. Joerg Landsmann
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  4. Francisco A.P. Campos
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Llamoca-Zárate, R.M., Studart-Guimarães, C., Landsmann, J. et al. Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell, Tissue and Organ Culture 58, 155–157 (1999). https://doi.org/10.1023/A:1006315729266

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  • DOI: https://doi.org/10.1023/A:1006315729266

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