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Cloning of Arabidopsis thaliana phosphatidylinositol synthase and functional expression in the yeast pis mutant

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Abstract

It is believed that phosphatidylinositol (PI) metabolism plays a central role in signalling pathways in both animals and higher plants. PI is synthesized from CDP-diacylglycerol (CDP-DG) and myo-inositol by phosphatidylinositol synthase (PI synthase, EC 2.7.8.11). Here we report the identification of a plant cDNA (AtPIS1) encoding a 26 kDa PI synthase from Arabidopsis thaliana. The plant enzyme as deduced from its cDNA sequence shares 35–41% identical amino acids with PI synthases from Saccharomyces cerevisiae and mammals. AtPIS1 functionally complements a mutant of S. cerevisiae with a lesion in PI synthase, and recombinant AtPIS1 protein present in yeast membranes strongly depends on the two principal substrates, myo-inositol and CDP-DG, and requires Mg2+ ions for full activity.

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Authors and Affiliations

  1. Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Am Mühlenberg 1, 14476, Golm/Potsdam, Germany

    Hong-Wei Xue, Gunnar Plesch & Bernd Mueller-Roeber

  2. Department of Basic Allied Medicine, Gunma University School of Health Sciences, Maebashi, 371-8514, Japan

    Kohei Hosaka

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  1. Hong-Wei Xue
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  2. Kohei Hosaka
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  3. Gunnar Plesch
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  4. Bernd Mueller-Roeber
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Xue, HW., Hosaka, K., Plesch, G. et al. Cloning of Arabidopsis thaliana phosphatidylinositol synthase and functional expression in the yeast pis mutant. Plant Mol Biol 42, 757–764 (2000). https://doi.org/10.1023/A:1006308909105

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