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Occurrence of cholinergic nerve fibers in the human uveoscleral tissue

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Abstract

We have studied the cholinergic nerve fibers (CNF) of human uveoscleral tissue in normal and pathological conditions (intraocular pressure: (IOP) of various degrees ) and have performed a quantitative image analysis of CNF with the aim of studying an hypothetical association between IOP and the uveoscleral level of cholinergic neurotransmitter in nerve fiber of the enucleated human eyes.

Eleven patients, previously enrolled in our medical protocols are affected by an increase of IOP of various degrees, were subjected to surgical enucleation of one eyeball for traumatic or neoplastic reasons and were the donors of samples of the uveoscleral tissue for CNF demonstration. The samples were also submitted to quantitative image analysis.

An enzymatic activity, Acetylcholinesterase (AChe), is present in nerve fibers that innervate the human uveoscleral tissue. The AChe-positive nerve fibers represent about 7.2% of the observed area in normal conditions, while in patients with elevated IOP ACheactivity is about 20.3% of the total observed area.

The increase of AChe activity in our experiments, all performed under the same conditions, and therefore, with comparable results, may be related with the increase of IOP.

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References

  1. Cavallotti C, Bucci MG, Pescosolido N. Enzymatic studies in the trabeculum of the dog. Methabol Ophthalmol 1977; 1: 219–222.

    Google Scholar 

  2. Gupta N, Drance SM, McAllister R, Prasad S, Rootman J, Cynader MS. Localization of muscarinic receptor subtype and mRNA in the human eye. Ophthalmic Res 1994; 26: 207–213.

    Article  PubMed  CAS  Google Scholar 

  3. Walkenbach RJ, Ye GS, Boney F, Duecker DK. Muscarinic cholinoreceptors in native and cultured human corneal endothelium. Curr Eye Res 1993; 12: 155–162.

    PubMed  CAS  Google Scholar 

  4. Lind GJ, Cavanagh HD. Identification and subcellular distribution of muscarinic acetylcholine receptor−related proteins in rabbit corneal and Chinese hamster ovary cells. Invest Ophthalmol Vis Sci 1995; 8: 1492–1507.

    Google Scholar 

  5. Lind GJ, Cavanagh HD. Nuclear muscarinic acetylcholine receptors in corneal cells from rabbit. Invest Ophthalmol Vis Sci 1993; 34: 2943–2952.

    PubMed  CAS  Google Scholar 

  6. Stone RA. Nervous System andintraocular pressure. In: R Ritch, MB Schields, T Krupin (eds). The Glaucomas Basic Sciences, Chap. 17. St. Louis: Mosby, 1996; pp. 357–383.

    Google Scholar 

  7. Ehinger B. Ocular and orbital vegetative nerves. Acta Physiol Scand 1996; 67: 1–29.

    Google Scholar 

  8. Laties AM, Jacobowitz D. A comparative study of autonomic innervation of the eye in monkey, cat and rabbit. Anat Rec 1996; 156: 383–396.

    Article  Google Scholar 

  9. Gerebtzoff MA. Recherches histochimiques sur les acetylcholine et cholinesterase I. Introduction et technique. Acta Anat 1953; 19: 366–378.

    Article  PubMed  CAS  Google Scholar 

  10. Koelle GM and Friedenwald US. Histochemical localisation of cholinesterase in ocular tissue. Am J Ophthalmol 1956; 33: 253–256.

    Google Scholar 

  11. More EJ, Fatty CS. A note of cholinesterase activity in postmortom tissues. J Histochem Cytochem 1958; 6: 377–379.

    Google Scholar 

  12. Manual of Methods of Quantimet Leica 500 Microsystems Imaging Solutions. Clifton Road Ltd., Cambridge, 1997.

  13. Prince JH. The Rabbit in Eye Research. Springfield: C. Thomas, 1964; pp. 126–139.

    Google Scholar 

  14. Kaufman PL, Barany EH. Loss of acute pilocarpine effect on outflow facility following surgical disinsertion and retrodisplacement of ciliary muscle from the scleral speron in the cynomolgus monkey. Invest Ophthalmol Vis Sci 1976; 15: 793–799.

    CAS  Google Scholar 

  15. Kater AW, Spun−Michaud SJ, Gipson JK. localization of smooth muscle myosin containing cells in the aqueous outflow pathway. Invest Ophthalmol Vis Sci 1990; 31: 347–356.

    PubMed  Google Scholar 

  16. Wolde Mussie H, Ruiz G, Feldman B. Muscarinic receptor subtype involved in signaling mechanism in cultured human trabecular meshwork cells ARVO: suppl. Invest Ophthalmol Vis Sci 1990; 31: 338–345.

    Google Scholar 

  17. Sugrue MF. The Pharmacology of antiglaucoma drugs. Pharm Therap 1989; 43: 91–138.

    Article  CAS  Google Scholar 

  18. Towsend DJ, Brubaker RF. Immediate effect of epinephrine on aqueous formation in the normal human eyes measured by fluorophotometry. Invest Ophthalmol Vis Sci 1980; 19: 256–268.

    Google Scholar 

  19. Lutjen−Drecoll E, Tamm E. Morfological study of the anterior segment of cynomolgus monkey eye following treatment with prostaglandin F. Exp Eye Res 1988; 47: 761–774.

    Article  PubMed  CAS  Google Scholar 

  20. Tamm E, Lutjen−Drecoll E, Ritting M. Connective tissues changes in the uveoscleral space pathways of primate eyes after treatment with prostaglandin F. ARVO: suppl. Invest Ophthalmol Vis Sci 1989; 30: 99; 113.

    Google Scholar 

  21. Bill A. Phillips CI. Uveoscleral drainage of aqueous humor in human eyes. Exp Eye Res 1971; 12: 275–289.

    Article  PubMed  CAS  Google Scholar 

  22. Inomata H, Bill A, Smelser GK. Unconventional routes of aqueous humor outflow in cynomolgus monkey (Macaca irus). Am J Ophthalmol 1972; 73: 893–902.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Cardiovascular and Respiratory Sciences and 3rd Chair of Human Anatomy, nUniversity `La Sapienza', Rome, Italy

    Carlo Cavallotti & Francesca Maria Tranquilli Leali

  2. Eye Clinic and 3rd Chair of Human Anatomy, University `La Sapienza', Rome, Italy

    Nicola Pescosolido & Francesco Gherardi

  3. 2nd Neurologic Clinic and 3rd Chair of Human Anatomy, University `La Sapienza', Rome, Italy

    Daniela Cavallotti

Authors
  1. Carlo Cavallotti
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  2. Nicola Pescosolido
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  3. Francesca Maria Tranquilli Leali
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  4. Francesco Gherardi
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  5. Daniela Cavallotti
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Cavallotti, C., Pescosolido, N., Leali, F.M.T. et al. Occurrence of cholinergic nerve fibers in the human uveoscleral tissue. Int Ophthalmol 22, 349–353 (1998). https://doi.org/10.1023/A:1006306812296

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