Plant Cell, Tissue and Organ Culture

, Volume 56, Issue 1, pp 17–23 | Cite as

Plant regeneration from seedling explants of Eucalyptus grandis × E. urophylla

  • Luis Pedro Barrueto CidEmail author
  • Adriane C.M.G. Machado
  • Sílvia B.R.C. Carvalheira
  • Ana Cristina M. Brasileiro


Hypocotyls, cotyledons, cotyledonary nodes and primary leaves were used as explants to establish a regeneration protocol for Eucalyptus grandis × E. urophylla. These seedling-derived explants were incubated on a modified MS medium (SP medium), supplemented with 2.0 μM TDZ. After 1 month, the calluses obtained were transferred to SP medium containing different concentrations of BA and NAA or zeatin and NAA. Shoots were induced from these calluses at a high frequency. Shoot elongation was then stimulated on SP medium supplemented with BA, NAA and GA3 for 20 to 30 days. For rooting, 50 mm long shoots were cultivated on root induction medium containing IBA (2.5 μM) for different periods and then transferred to the same medium but without auxin, for 30 days. Plantlets were then successfully transplanted to greenhouse conditions.

Eucalyptus organogenesis shoot induction thidiazuron 


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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Luis Pedro Barrueto Cid
    • 1
    Email author
  • Adriane C.M.G. Machado
    • 1
  • Sílvia B.R.C. Carvalheira
    • 1
  • Ana Cristina M. Brasileiro
    • 1
  1. 1.Área de Biologia Celular – Cenargen/EmbrapaBrasília-DFBrazil requests for offprints; fax

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