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Improvements in shoot apex regeneration of two fiber crops: cotton and kenaf

Abstract

Cotton (Gossypium hirsutum L.) and kenaf ( Hibiscus cannabinus L.) belong to the Malvaceae family, and both are used as sources of fibers. Shoot apices from vigorous seedlings aseptically germinated from 3 different cultivars of both cotton and kenaf were used in this study. The cotton and kenaf shoot apex size was between 2–3 mm containing the meristem, unexpanded leaves, and a small portion of the cotyledon. Shoot apices were placed on 18 different media comparing full and 1/2 strength Murashige and Skoog (1962) plus vitamins, and combinations of 0, 0.1, and 1 mg l-1 of naphthaleneacetic acid and 6-benzyladenine (BA). The shoot apices of both crops developed successfully without intervening callus formation, and no significant differences among cultivars were found. An average of 58% of the cotton shoot apices initiated shoot and rooted in full strength Murashige and Skoog (1962) plus vitamins in 6 weeks. For kenaf, an average of 92% of shoot apices initiated shoot and rooted in full strength Murashige and Skoog plus vitamins and 0.1 mg l-1 BA in 3 weeks. All regenerated plants of both crops were phenotypically normal and set seeds.

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Correspondence to Roberta H. Smith.

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Zapata, C., Srivatanakul, M., Park, SH. et al. Improvements in shoot apex regeneration of two fiber crops: cotton and kenaf. Plant Cell, Tissue and Organ Culture 56, 185–191 (1999). https://doi.org/10.1023/A:1006238924439

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  • DOI: https://doi.org/10.1023/A:1006238924439

  • Malvaceae
  • Gossypium
  • Hibiscus
  • plant regeneration
  • shoot apical meristem
  • rooting