Skip to main content
SpringerLink
Log in

Effect of activated charcoal, autoclaving and culture media on sucrose hydrolysis

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The effect of activated charcoal, autoclaving and culture media on sucrose hydrolysis in tissue culture media was investigated. Activated charcoal acidified an aqueous sucrose (5%) solution and culture media by about 1 to 2 units after autoclaving. Sucrose hydrolysis in tissue culture media and/or aqueous sucrose (5%) solutions containing activated charcoal (buffered to pH 5.8) was dependent on both the hydrogen ion concentration (pH) and autoclaving. After autoclaving, 70%, 56% and 53% sucrose hydrolysis were respectively recorded in a 5% sucrose solution, Murashige and Skoog (MS) and Gamborg B5 (B5) liquid media in the presence of 1% activated charcoal, added before autoclaving. In the absence of activated charcoal, autoclaving resulted in about 20% of the sucrose being hydrolyzed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Anagnostakis SL (1974) Haploid plants from anthers of tobacco: enhancement with charcoal. Planta 115: 281–283

    Google Scholar 

  2. Ball E (1953) Hydrolysis of sucrose by autoclaving media, a neglected aspect in the technique of culture of plant tissue. Bull Torrey Bot Club 80: 409–411

    Google Scholar 

  3. Borkowska B and Szczerba J (1991) Influence of different carbon sources on invertase activity and growth of sour cherry (Prunus cerasus L.) shoot cultures. J Exp Bot 42: 911–915

    Google Scholar 

  4. Constantin MJ, Henke RR and Mansur MA (1977) Effect of activated charcoal on callus growth and shoot organogenesis in tobacco. In vitro 13: 293–287

    Google Scholar 

  5. De Lange JH (1989) Significance of autoclaving-induced toxicity from hydrolysis of carbohydrates in in vitro studies of pollen germination and tube growth. S Afr J Bot 55: 1–5

    Google Scholar 

  6. Drew RA (1992) Improved techniques for the in vitro propagation and germplasm storage of papaya. HortScience 27: 1122–1224

    Google Scholar 

  7. Druart P and De Wulf O (1993) Activated charcoal catalyses sucrose hydrolysis during autoclaving. Plant Cell Tissue Org Cult 32: 97–99

    Google Scholar 

  8. Dumas E and Monteuuis O (1995) In-vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants - influence of activated charcoal. Plant Cell Tissue Org Cult 40: 231–235

    Google Scholar 

  9. Ernst (1975) Studies in asymbiotic culture of orchids. Amer Orchid Soc Bul 44: 12–18

    Google Scholar 

  10. Fridborg G, Pedersen M, Landstrom and Eriksson T (1978) The effect of activated charcoal on tissue cultures: adsorption of metabolites inhibiting morphogenesis. Physiol Plant 43: 104–106

    Google Scholar 

  11. Fridborg G and Eriksson T (1975) Effects of activated charcoal on morphogenesis in plant tissue cultures. Physiol Plant 34: 306–308

    Google Scholar 

  12. Gamborg OL, Miller RA and Ojina K (1968) Nutritional requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–258

    Google Scholar 

  13. Hine J (1962) Physical Organic Chemistry. New York: McGraw Hill Publishing Co., pp 120

    Google Scholar 

  14. Johansson L, Galleberg E and Gedin A (1990) Correlation between activated charcoal, Fe EDTA and other organic media ingredients in cultures of anthers of Anemone canadensis. Physiol Plant 80: 243–249

    Google Scholar 

  15. Levi A and Sink KC (1990) Differential effects of sucrose, glucose and fructose during somatic embryogenesis in asparagus. J Plant Physiol 137: 184–189

    Google Scholar 

  16. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  17. Nash DT and Boll WG (1975) Carbohydrate nutrition of Paul's Scarlet rose cell suspensions. Can J Bot 53: 179–185

    Google Scholar 

  18. Nissen SJ and Sutter EG (1990) Stability of IAA and IBA in nutrient medium to several tissue culture procedures. Hort-Science 25: 800–802

    Google Scholar 

  19. Owen HR, Wengerd D and Miller AR (1991) Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method. Plant Cell Rep 10: 583–586

    Google Scholar 

  20. Sweeley CC, Bentley R, Makita M and Wells WW (1963) Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J Amer Chem Soci 85: 2497–2507

    Google Scholar 

  21. Tanowitz BD and Smith DM (1984) A rapid method for qualitative analysis of simple carbohydrates in nectars. Ann Bot 53: 453–463

    Google Scholar 

  22. Wann SR, Veazey RL and Kaphammer J (1997) Activated charcoal does not catalyze sucrose hydrolysis in tissue culture media during autoclaving. Plant Cell Tissue Org Cult 50: 221–224

    Google Scholar 

  23. Weatherhead MA, Burdon J and Henshaw GG (1978) Some effects of activated charcoal as an additive to plant tissue culture media. Z Pflanzenphysiol 89: 141–147

    Google Scholar 

  24. Wickremesinhe ER M and Arteca RN (1994) Taxus cell suspension cultures: Optimizing growth and production of taxol. J Plant Physiol 144: 183–188

    Google Scholar 

  25. Yu XL and Reed BM (1993) Improved shoot multiplication of mature hazelnut (Corylus avellana L.) in vitro using glucose as a carbon source. Plant Cell Rep 12: 256–259

    Google Scholar 

  26. Zaghmout OMF and Torello WA (1988) Enhanced regeneration in long-term callus culture of red fescue by pretreatment with activated charcoal. HortScience 23: 615–616

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of Natal Pietermaritzburg, University of Natal Research Unit for Plant Growth and Development, Private Bag X01, Scottsville 3209, Republic of South Africa

    M.J. Pan & J. van Staden

Authors
  1. M.J. Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. van Staden
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pan, M., Staden, J.v. Effect of activated charcoal, autoclaving and culture media on sucrose hydrolysis. Plant Growth Regulation 29, 135–141 (1999). https://doi.org/10.1023/A:1006238300890

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006238300890

Search

Navigation