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A cell cycle role for a plant sucrose nonfermenting-1-related protein kinase (SnRK1) is indicated by expression in yeast

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Abstract

Plant sucrose nonfermenting-1 (SNF1)-related protein kinases (SnRK1s) have been shown to restore carbon catabolite derepression of gene expression in the yeast Saccharomyces cerevisiae when expressed in snf1 mutants. SNF1 has been implicated in the mediation of cell cycle control in response to nutrient levels and, in the present study, we show that expression of the rye (Secale cereale) SnRK1, RKIN1, in a yeast snf1 mutant has a dramatic effect on the size of cells growing on a minimal medium where SNF1 function is essential. The mean volume of the yeast cells which were expressing RKIN1 was two-thirds that of the whi1 mutant, the smallest viable cells known in S. cerevisiae, and the cells died after 3 days unless rescued onto complex medium. This is the first experimental evidence of a role for SnRK1s in plant cell cycle control.

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References

  1. Alderson A, Sabelli PA, Dickinson JR, Cole D, Richardson M, Kreis M, Shewry PR and Halford NG (1991) Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA. Proc Natl Acad Sci USA 88: 8602–8605

    Google Scholar 

  2. Aon MA and Cortassa S (1999) Quantitation of the effects of disruption of catabolite (de)repression genes on the cell cycle behaviour of Saccharomyces cerevisiae. Curr Microbiol 38: 57–60

    Google Scholar 

  3. Gancedo JM (1998) Yeast carbon catabolite repression. Microbiol Mol Biol Rev 62: 334–361

    Google Scholar 

  4. Genetics Computer Group (1994) Program Manual for the Wisconsin Package, Version 8. Madison, Wisconsin: University of Wisconsin

    Google Scholar 

  5. Halford NG and Hardie DG (1998) SNF1-related protein kinases: global regulators of carbon metabolism in plants? Plant Mol Biol 37: 735–748

    Google Scholar 

  6. Hardie DG, Carling D and Halford NG (1994) Roles of the Snf1/Rkin1/AMP-activated protein kinase family in the response to environmental and nutritional stress. Seminars Cell Biol 5: 409–416

    Google Scholar 

  7. Hardie DG and Carling D (1997) The AMP-activated protein kinase: fuel gauge of the mammalian cell? Eur J Biochem 246: 259–273

    Google Scholar 

  8. Johnston M and Carlson M (1992) Regulation of carbon and phosphate utilization. In: Jones EW, Pringle JR and Broach JR (eds) The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression. Cold Spring Harbor, U.S.A.: Cold Spring Harbor Laboratory, pp 193–281

    Google Scholar 

  9. Muranaka T, Banno H and Machida Y (1994) Characterization of the tobacco protein kinase NPK5, a homologue of Saccharomyces cerevisiae SNF1 that constitutively activates expression of the glucose-repressible SUC2 gene for a secreted invertase of S. cerevisiae. Mol Cell Biol 14: 2958–2965

    Google Scholar 

  10. Purcell PC, Smith AM and Halford NG (1998) Antisense expression of a sucrose nonfermenting-1-related protein kinase sequence in potato results in decreased expression of sucrose synthase in tubers and loss of sucrose-inducibility of sucrose synthase transcripts in leaves. Plant J 14: 195–202

    Google Scholar 

  11. Ronne H (1995) Glucose repression in fungi. Trends in Genetics 11: 12–17

    Google Scholar 

  12. Russell P and Nurse P (1987) The mitotic inducer nim1 + functions in a regulatory network of protein kinase homologs controlling initiation of mitosis. Cell 49: 569–576

    Google Scholar 

  13. Sudbery PE, Goodey AR and Carter BLA (1980) Genes which control cell proliferation in the yeast Saccharomyces cerevisiae. Nature 288: 401–404

    Google Scholar 

  14. Tanaka S and Nojima H (1996) Nik1: a Nim1-like protein kinase of S. cerevisiae interacts with the Cdc28 complex and regulates cell cycle progression. Genes to Cells 1: 905–921

    Google Scholar 

  15. Thompson-Jaeger S, Francois J, Gaughran JP and Tatchell K (1991) Deletion of SNF1 affects the nutrient response of yeast and resembles mutations which activate the adenylate cyclase pathway. Genetics 129: 697–706

    Google Scholar 

  16. Tibbetts M, Donovan M, Roe S, Stiltner AM and Hammond CI (1994) KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane. Exp Cell Res 213: 93–99

    Google Scholar 

  17. Woods A, Munday MR, Scott J, Yang X, Carlson M and Carling D (1994) Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl CoA carboxylase in vivo. J Biol Chem 269: 19509–19515

    Google Scholar 

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Authors and Affiliations

  1. Cardiff School of Biosciences, Cardiff University, Cardiff, CF1 3TL, U.K.

    J. Richard Dickinson & Deborah Cole

  2. IACR-Long Ashton Research Station, Department of Agricultural, Sciences University of Bristol, Long Ashton, Bristol, BS41 9AF, U.K

    Nigel G. Halford

Authors
  1. J. Richard Dickinson
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  2. Deborah Cole
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  3. Nigel G. Halford
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Correspondence to J. Richard Dickinson.

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Dickinson, J.R., Cole, D. & Halford, N.G. A cell cycle role for a plant sucrose nonfermenting-1-related protein kinase (SnRK1) is indicated by expression in yeast. Plant Growth Regulation 28, 169–174 (1999). https://doi.org/10.1023/A:1006222425228

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  • DOI: https://doi.org/10.1023/A:1006222425228

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