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In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae)


Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 μM thidiazuron, 4.44 μM 6-benzylaminopurine and 0.05 μM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 μM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions.

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Correspondence to G. Bringmann.

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Bringmann, G., Rischer, H., Schlauer, J. et al. In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae). Plant Cell, Tissue and Organ Culture 57, 71–73 (1999).

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  • liana
  • micropropagation
  • naphthylisoquinoline alkaloids
  • tissue culture