Abstract
The in vivo toxic impact of chromium in its two forms(trivalent and hexavalent) on glycogen metabolism in the tissuesof freshwater field crab Barytelphusa guerini was studied.In a sublethal long-term exposure of 30, 60 and 90 days, thedepletion of glycogen and glucose levels reflected in theactivity of glycogenphosphorylases `a' and `ab'. A tissuespecific and time-dependent variation in tissue glucose reservesparelleled the progressive accumulation of haemolymph sugars.Trivalent and hexavalent chromium induced alterations in metabolite levels and in enzyme activities in the tissues. Themagnitude of responses were found more in the hexavalent formthan in trivalent form.
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Anderson, A. R., Polansky, M. M., Bryden, N. A., Roginski, E. E., Mertz, W. and Glinsmann, W.: 1983, ‘Chromium supplementation of human subjects: Effects on glucose, insulin and lipid variables’, Metabolism 32, 894–899.
Baetjer, A. H., Birmighm, D. J., Enterline, P. E., Mertz, W. and Pierce, J. O.: 1974, Chromium, National Academy of Sciences, Washington D.C.
Bagyalaxmi, A., Reddy, P. S. and Ramamurthy, R.: 1984, Toxicol. Lett. 21, 127.
Cori, G. T., Illingworth, B. and Keller, P. J.: 1955, ‘Muscle Phosphorylase’, in: S. P. Colowick and N. O. Kaplan (eds.), Methods in Enzymology, Vol. 1, Academic Press, New York, p. 200.
Dezwaan, A. and Zandee, D. I.: 1972, ‘The utilization of glycogen and accumulation of some intermediates during anaerobiosis in Mytilus edulis’, Comp. Biochem. Physiol. 43, 47–54.
Doughtie, D. and Rao, R. K.: 1984, ‘Histopathological and ultrastructural changes in the antennal gland, midgut, hepatopancreas and gill of grass shrimp Palemonetes pungio, following exposure to hexavalent chromium’, J. Invertebr. Pathol. 43, 89–108.
Dugan, P. R.: 1972, in Biochemical Ecology of Water Pollution, Plenum Press, New York.
Eastin Jr., W. C., Jaseltine, S. D. and Murray, H. C.: 1980, ‘Intestinal Absorption of 5 Chromium Compounds in Young Black Ducks’, Toxicol Lett. 6, 193–197.
Finney, D. J.: 1964, Probit Analysis, 2nd Ed., Cambridge University Press, London.
Ghafghazi, T., Maghbareh, A. and Barnett, R.: 1979, ‘Chromium Induced Hyperglycemia in the Rat’, Toxicology 12, 47–52.
Lockwood, A. P. M.: 1968, Aspects of Physiology of Crustacea, Oliver and Boyer Ltd., London, 328.
Lowry, O. H., Rosebrough, J., Farr, A. L. and Randell, R. J.: 1951, ‘Protein Measurement with the Folin Reagent’, J. Biol. Chem. 193, 265–275.
Mertz, W.: 1969, ‘Chromium occurrence and function in biological system’, Physiol. Rev. 49, 163–239.
Nicholas, V. C., Longley, R. W. and Row, J. H.: 1956, ‘Determination of Glycogen in Liver and Muscle by use of Anthrone Reagent’, J. Biol. Chem. 110, 583–593.
Rajaram, Rama, Balachandran, Unninair and Rama Swami: 1995, ‘Chromium(III) inducedabnormalities in human lymphocyte cell proliferation’, Biochem. and Biophys. Research Com. 210, 434–440.
Reddy, S. L. N., Venugopal, N. B. R. K. and Ramana Rao, J. V.: 1989, ‘In vivo effects of cadmium chloride on certain aspects of carbohydrate metabolism in the issue of fresh water field crab Barytelphusa guerini’, Bull. Environ. Contam. Toxicol. 42, 857–866.
Saravannan, T. S. and Ravindranath, M. H.: 1981, ‘Total Free Sugars, Reducing Sugar and Glucose’, in Manual of Research Methods for Crustacean Biochemistry and Physiology, pp. 17–21.
Sastry, K. V. and Sunita, K.: 1982, ‘Toxic Effects of Chromium in a FreshWater Telecost Fish Canna punctatus’, Toxicol. Lett. 11, 17–21.
Sastry, K. V. and Sunita, K.: 1983, ‘Enzymological and biochemical changes produced by chromic chromium exposure in a teleost fish Channa punctatus’, Toxicol. Lett. 16, 9–15.
Sather, B. T.: 1967, ‘Chromium adsorption and metabolism by the crab Podopthehalmus vigil’, Pergamon Press, Symposium Publications Division, London and New York, 943–976.
Shivakumar, K., Pande, A. K. and Biksham, G.: 1997, ‘Toxic trace element pollution in ground water around Patancheruvu and Bolaram industrial areas, A. P. India-A graphical approach’, Environ. Monit. Asses. 45, 57–80.
Venugopal, N. B. R. K.: 1989, ‘Metabolic alterations during trivalent and hexavalent Chromium intoxication in a fresh water fish Anabas scandens’, Ph.D. Thesis, Osmania University, Hyderabad, India.
Venugopal, N. B. R. K. and Reddy, S. L. N.: 1992, ‘Nephrotoxic and hepatotoxic effects of hexavalent and trivalent chromium in a fresh water teleost Anabas scandens, biochemical and environmental changes’, Ecotoxicol. Environ. Safety 24, 287–293.
Varghese, G., Prashant, S. N. and Meena, K.: 1991, ‘Respiratory responses and blood sugar level of the crab Cunicularis exposed to mercury, copper and zinc’, Indian J. Experimen. Biol., 30, 308–312.
Zey, J. N. and AW, T. C.: 1984, American transportation corp. (Health hazard evaluation report No. 82–025–1413) Cincinnati, OH. National institute for occupational safety and health.
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Sridevi, B., Reddy, S.L.N. Effect of Trivalent and Hexavalent Chromium on Carbohydrate Metabolism of a Freshwater Field Crab, Barytelphusa guerini. Environ Monit Assess 61, 293–302 (2000). https://doi.org/10.1023/A:1006198127933
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DOI: https://doi.org/10.1023/A:1006198127933