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A novel flower-specific Arabidopsis gene related to both pathogen-induced and developmentally regulated plant β-1,3- glucanase genes

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Abstract

β-1,3-glucanases are usually associated with plant defense responses, although some are also developmentally or hormonally regulated. We characterized two Arabidopsis genes linked in a tandem array, BG4 and BG5, encoding putative novel isoforms of β-1,3-glucanase. The deduced polypeptides, BG4 and BG5, were highly similar to each other (89% amino acid identity) but only moderately related (32 to 41% amino acid identity) to the different categories of previously characterized β-1,3-glucanases, suggesting that BG4 and BG5 may represent a novel class of β-1,3-glucanases in plants. Neither of the genes was responsive to pathogen or SA induction in contrast to the previously identified Arabidopsis β-1,3-glucanases, nor could we detect any developmental or hormonally induced expression in the vegetative parts of the plants. Both RNA blot and in situ hybridization data demonstrated that the BG4 gene was specifically expressed in the style and septum of the ovary, suggesting that the corresponding protein is involved in the reproductive process of the plant.

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Authors and Affiliations

  1. Department of Plant Science, Waite Campus, University of Adelaide, Glen Osmond, South Australia, 5064, Australia

    Gabriele Delp

  2. Department of Biosciences, Division of Genetics, University of Helsinki, Box 56, Helsinki, Finland

    E. Tapio Palva

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  1. Gabriele Delp
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  2. E. Tapio Palva
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Delp, G., Palva, E.T. A novel flower-specific Arabidopsis gene related to both pathogen-induced and developmentally regulated plant β-1,3- glucanase genes. Plant Mol Biol 39, 565–575 (1999). https://doi.org/10.1023/A:1006194822666

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