Abstract
Poplar shoots raised in vitro were induced to root by incubation on an auxin (NAA) containing medium for 7 h. After 13 days on an auxin-free medium, 97% of the treated shoots had rooted. The introduction of known antiauxins (PCIB, PBA, POAA) into the rooting expression auxin-free medium, after the 7-h induction by NAA, completely (PCIB and PBA) or severely (POAA) inhibited rooting. The exclusion of calcium from the expression auxin free medium reduced the percentage of rooting by about 42%. The inhibition was still higher in the presence of EGTA, a calcium chelator. Lanthanum chloride, a calcium channel blocker, also completely inhibited rooting, when incorporated into the auxin free medium, with or without calcium. These results support previous hypotheses about the implication of both endogenous auxin and calcium in the late phases of the adventitious rooting process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bellamine J and Gaspar T (1998) The relationship between rooting of poplar shoots and ATPase activity of their crude microsomal vesicles. Plant Growth Regul 24: 43-48
Bellamine J, Penel C and Greppin H (1993) Proton pump and IAA sensitivity changes in spinach leaves during the flowering induction. Plant Physiol Biochem 31: 197-203
Berthon JY, Ben Tahar S, Gaspar T and Boyer N (1990) Rooting phases of shoots of Sequoiadendron giganteum in vitro and their requirements. Plant Physiol Biochem 28: 631- 638
Blakesley D, Weston GD and Hall JF (1991) The role of endogenous auxin in root initiation. Part I. Evidence from studies on auxin application and analysis of endogenous levels. Plant Growth Regul 10: 341-353
Blazich FA (1988) Mineral nutrition and adventitious rooting. In: Davis TD, Haissig B and Sankhla N (eds) Adventitious Root Formation in Cuttings. Portland: Dioscorides Press, pp 61-69
Davies PJ (1995) Plant Hormones. Physiology, Biochemistry and Molecular Biology (2nd edn). Dordrecht: Kluwer Academic Publishers, 833 pp
Druart P (1997) Optimization of culture media for in vitro rooting of Malus domestica Borkh. cv. Compact Spartan. Biol Plant 39: 67-77
Eliasson L (1978) Effects of nutrients and light on growth and root formation in Pisum sativum cuttings. Physiol Plant 43: 13-l8
Gaspar T and Coumans M (1987) Root formation. In: Bonga JM and Durzan DJ (eds) Cell and Tissue Culture in Forestry. Vol 2. Specific Principles and Methods: Growth and Developments. Dordrecht: Martinus Nijhoff Publishers, pp 202-217
Gaspar T and Hofinger M (1988) Auxin metabolism during adventitious rooting. In: Haissig B and Sankhla N (eds) Adventitious Root Formation in Cuttings. Portland: Dioscorides Press, pp 117-131
Gaspar T Kevers C and Hausman JF (1997) Indissociable chief factors in the inductive phase of adventitious rooting. In: Altman A and Waisel Y (eds) Biology of Root Formation and Development. New York: Plenum Press, pp 55-63
Gaspar T, Kevers C, Hausman JF and Ripetti V (1994) Peroxidase activity and endogenous free auxin during adventitious root formation. In: Lumsden PJ, Nicholas JR and Davies WJ (eds) Physiology, Growth and Development of Plants in Culture. Dordrecht: Kluwer Academic Publishers, pp 289-298
Hausman JF, Evers D, Kevers C and Gaspar T (1997) Internal controls of root induction in poplar shoots raised in vitro. J Appl Bot/Angew Bot 71: 104-107
Hausman JF, Kevers C and Gaspar T (1995) Auxin-polyamine interaction in the control of the rooting inductive phase of poplar shoots in vitro. Plant Sci 110: 63-71
Imaseki H (1985) Hormonal control of wound-induced responses. In: Pharis RP and Reid DM (eds) Hormonal Regulation of Development III. Encycl Plant Physiol. Berlin: Springer-Verlag, pp 485-512
Jarvis BC (1986) Endogenous control of adventitious rooting in non woody cuttings. In: Jackson MB (ed) New Root Formation in Plants and Cuttings. Dordrecht: Martinus Nijhoff Publishers, pp 191-222
Jay-Allemand C, Capelli P and Cornu D (1992) Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing gelrite medium. Sci Hort 51: 335-342
Kevers C, Hausman JF, Faivre-Rampant O, Evers D and Gaspar T (1997) Hormonal control of adventitious rooting: Progress and questions. J Appl Bot/Angew Bot 71: 71-79
Marumo S (1986) Auxins. In: Takahashi N (ed) Chemistry of Plant Hormones. Boca Raton: CRC Press, pp 9-56
Mengel K and Kirkby EA (1982) Principles of Plant Nutrition. Intl Pot Inst Bern: Worblaufen
Moncousin C, Favre JM and Gaspar T (1988) Changes in peroxidase activity and endogenous 1AA levels during adventitious rooting in vine cutiings. In: Kutacek M, Bandurski RS and Krekule J (eds) Physiology and Biochemistry of Auxins in Plants. Praha: Academia, pp 331-337
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassavs with tobacco tissue culture. Physiol Plant 15: 473-497
Pharis RP and Reid DM (1985) Hormonal Regulation of Development III. Role of Environment Factors. Encycl Plant Physiol 11. Berlin: Springer Verlag, 887 pp
Ripetti V, Kevers C and Gaspar T (1994) Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and in ethylene production. Adv Hortic Sci 8: 29-32
Roblin G, Fleurat-Lessard P and Bonmort J (1989) Effects of compounds affecting calcium channels on phytochrome and blue pigment-mediated pulvinar movements of Cassia fasciculata. Plant Physiol 90: 697-701
Santoni V, Vansuyt G and Rossignol M (1991) The changing sensitivity to auxin of the plasma membrane HC-ATPase: Relationship between plant development and ATPase content of membranes. Planta 185: 227-232
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bellamine, J., Penel, C., Greppin, H. et al. Confirmation of the role of auxin and calcium in the late phases of adventitious root formation. Plant Growth Regulation 26, 191–194 (1998). https://doi.org/10.1023/A:1006182801823
Issue Date:
DOI: https://doi.org/10.1023/A:1006182801823