Abstract
Day-old broiler chicks, which had been shown to be negative for maternal antibodies against inclusion body hepatitis (IBH) virus and for viral antigen in cloacal swabs, were divided into four groups of 20 chicks each. One group was fed ochratoxin-A at 0.5 ppm from 3 to 38 days of age, another group was inoculated with 1 ml of IBH virus containing 106.5 EID50 per 0.2 ml. A third group was given both ochratoxin-A and infected with IBH virus. The fourth group served as the control. Anaemia was observed in all three treated groups but the changes were more pronounced in the combined group. The biochemical changes also suggested a cumulative damaging effect by ochratoxin-A and IBH virus.
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REFERENCES
Benzamin, M.M., 1985. Outlines of Veterinary Clinical Pathology, 3rd edn, (Kalyani Publishers, New Delhi)
Bickford, A.A., 1972. Inclusion body hepatitis in chickens. Poultry Digest, 31, 345-347
Burns, R.R. and Dwivedi, P., 1986. The natural occurrence of ochratoxin A and its effects in poultry. A review. Pathology and Immunology. World Poultry Science Journal, 42, 32-47
Doerr, J.A., Huff, W.E., Tung, H.T., Wyatt, R.D. and Hamilton, P.B., 1974. A survey of T-2 toxins ochratoxin, aflatoxin for their effects on the coagulation of blood in young broiler chickens. Poultry Science, 53, 1728-1734
Doerr, J.A., Huff, W.E., Hamilton, P.B. and Lillehoj, E.B., 1981. Severe coagulopathy in young chickens produced by ochratoxin A. Toxicology and Applied Pharmacology, 59, 157-163
Doupnik, B. and Peckham, J.C., 1970. Mycotoxicity of Aspergillus ochraceus to chicks. Journal of the American Oil Chemists Society, 46, 122A
Dwivedi, P., 1984. The immunological and pathological changes in poultry induced by OA, (PhD thesis, University of Edinburgh)
Dwivedi, P. and Burns, R.B., 1986. The natural occurrence of ochratoxin A and its effects in poultry. A review. Epidemiology and toxicology. World Poultry Science Journal, 42 32-47
Elling, F., Hald, B., Jacobsen, C. and Krogh, P., 1975. Spontaneous toxic nephropathy in poultry, associated with OA. Acta Pathologica etMicrobiologica Scandinavica, Section-A, 83, 739-741
Hald, B. and Krogh, P., 1975. Detection of ochratoxin A in barley, using silica gel minicolumns. Journal of the Association of Official Analytical Chemists, 58, 156-158
Hamilton, P.B., Huff, W.E., Harris, J.R. and Wyalt, R.D., 1982. Natural occurrence of ochratoxicosis in poultry. Poultry Science, 61, 1832-1841
Hoffman, R. and Dorn, P., 1978. Histological development of lesions in the bursa of Fabricius of chickens with inclusion body hepatitis. Avian Diseases, 22, 266-272
Huff, W.E. and Hamilton, P.B., 1974. Nephrotoxicity of ochratoxin A in broiler chicks. Poultry Science, 62, 1764-1771
Huff, W.E., Yatt, R.D. and Hamilton, P.B., 1975. Nephrotoxicity of dietary ochratoxin A in broiler chickens. Applied Microbiology, 138, 48-51
Huff, W.E., Doerr, J.A. and Hamilton, P.B., 1979. Decreased glycogen mobilization during ochratoxicosis in broiler chickens. Applied and Environmental Microbiology, 37, 122-126
Huff, W.E., Kubena, I.F. and Harvey, R.B., 1988. Progression of ochratoxicosis in broiler chickens. Poultry Science, 67, 1139-1146
Itakura, C., Yasuba, M. and Goto, M., 1974. Histopathological studies on inclusion body hepatitis in broiler chickens. Japanese Journal of Veterinary Science, 36, 329-340
Kohler, H. and Hromatka, L., 1974. Inclusion body hepatitis in broilers in Austria (Preliminary communication). Weiner Tierarztliche Monatsschrift, 61, 90-94
Manning, R.O. and Wyatt, R.D., 1984. Toxicity of Aspergillus ochraceus-contaminated wheat and different chemical forms of ochratoxin A in broiler chicks. Poultry Science, 63, 458-465
Mishra, S.K., 1988. Studies on the prevalence and pathology of inclusion body hepatitis in broiler chicks, (PhD thesis, Punjab Agricultural University, Ludhiana)
Naqi, S.A., Adams, L.G., Panigraphy, B. and Vivek, A.R., 1978. Experimental induction of haemorrhagic aplastic anaemia in chickens. I. Etiology. Avian Diseases, 22, 675-682
Nayak, M.C., Chakrabarti, K., Chakrabarti, S. and Chakrabarti, A., 1990. An outbreak of inclusion body hepatitis in broiler chickens inWest Bengal. Indian Veterinary Journal, 67, 7-9
Oberoi, M.S., Ramneek Maiti, N.K. and Sharma, S.N., 1993. A rapid indirect DOT enzyme immunoassay for direct detection of viruses. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 3&4, 1-3
Panisup, A.S., Mohanty, G.C. and Verma, K.C., 1982. Spontaneous occurrence of inclusion body hepatitis syndrome in immunosuppressed chickens. Indian Journal of Veterinary Pathology, 6, 24-28
Peckham, J.C., Doupnik, B. and Jones, H.O., 1971. Acute toxicity of OA and OB in chicks. Applied Microbiology, 21, 492-494
Prior, M.C. and Sisodia, C.S., 1978. Ochratoxicosis inWhite Leghorn hens. Poultry Science, 57, 619-623
Raina, J.S., 1989. Prevalence and pathology of mycotoxicosis in poultry in Punjab, (MVSc thesis, Punjab Agricultural University, Ludhiana)
Reece, R.L., Grix, D.C. and Barr, D.A., 1986. An unusual case of inclusion body hepatitis in a cockrel. Avian Diseases, 30, 224-227
Sandhu, B.S., Singh, H. and Singh, B., 1995. Pathological studies in broiler chicks fed aflatoxin or ochratoxin and inoculated with inclusion body hepatitis virus, singly and in concurrence. Veterinary Research Communications, 19, 27-37
Schalm, O.W., Jain, N.C. and Corroll, E.J., 1975. Veterinary Hematology, 3rd edn., (Lea and Febiger, Philadelphia)
Singh, A., Oberoi, M.S., Jand, S.K. and Singh, B., 1996. Epidemiology of inclusion body hepatitis in poultry in northern India from 1990 to 1994. Revue Scientifique et Technique de l'Office International des Epizooties, 15, 1053-1060
Snedecor, G.W. and Cochran, W.G., 1967. Statistical Methods, 6th edn., (Oxford and IBH, Calcutta)
Van Der Merwe, K.J., Steyn, P.S., Fourie, I., Scott, D.B. and Theron, J.J., 1965. Ochratoxin, a toxic metabolite produced by Aspergillus ochraceus wilh. Nature, 205, 1112-1113
Warren, M.F. and Hamilton, P.B., 1980. Inhibition of the glycogen phosphorylase system during ochratoxicosis in chickens. Applied Environment and Microbiology, 40, 522-525
Wiseman, B.K., 1931. An improved method for obtaining white cell count in avian blood. Proceedings of Experimental Biological Methods, 28, 1030-1033
Woernle, H., 1966. The use of agar gel diffusion technique in identification of certain avian virus diseases. The Veterinarian, 4, 17-28
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Sandhu, B., Singh, B. & Brar, R. Haematological and biochemical studies in broiler chicks fed ochratoxin and inoculated with inclusion body hepatitis virus, singly and in concurrence. Vet Res Commun 22, 335–346 (1998). https://doi.org/10.1023/A:1006177222023
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DOI: https://doi.org/10.1023/A:1006177222023