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Alleletyping of an Oligodendrocyte-type-2 Astrocyte Lineage Derive from a Human Glioblastoma Multiforme

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Abstract

We have conducted alleletyping of two novel cell lines derived from glioblastoma multiforme, which appear to have arisen from different glial lineages, by using 76 fluorescently labeled oligonucleotide primers amplifying microsatellite loci covering the entire human genome. One cell line, Hu-O-2A/Gb1, expresses antigens and metabolic profiles characteristic of the oligodendrocyte-type-2 astrocyte (0-2A) lineage of the rat central nervous system. This cell line generated, in vitro, cells with characteristics of 0-2A progenitor cells, oligodendrocytes and astrocytes. The second cell line, IN1434, is derived from an astrocyte or a precursor cell restricted to astrocytic differentiation. Hu-O-2A/Gb1 cells show allelic losses of loci on chromosomes 2, 5, 6, 7, 8, 9, 10, 11, 13, 15, 16, 17, 20 and 21. IN1434 cells are likely to have allelic losses of loci on chromosomes 1, 3, 8 and 10, although no control DNA is available for this cell line. These results, for the first time, provide a detailed information of the molecular genetic defects occurring in Hu-O-2A/Gb1 and IN1434.

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Authors and Affiliations

  1. Human Cytogenetics Laboratory, Imperial Cancer Research Fund, London, UK

    Xin Mao

  2. Section of Molecular Carcinogenesis, Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, UK

    Xin Mao, Rita Barfoot & Rifat A. Hamoudi

  3. Ludwig Institute for Cancer Research, London, UK

    Mark Noble

  4. Huntsman Cancer Institute, University of Utah Health Sciences Center, Biopolymers Research Building 570, Salt Lake City, UT, USA

    Mark Noble

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  1. Xin Mao
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Mao, X., Barfoot, R., Hamoudi, R.A. et al. Alleletyping of an Oligodendrocyte-type-2 Astrocyte Lineage Derive from a Human Glioblastoma Multiforme. J Neurooncol 40, 243–250 (1998). https://doi.org/10.1023/A:1006158010388

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