Abstract
Mung bean hypocotyl cuttings were treated with indole-3-butyric acid (IBA), 3-(benzo[b]selenienyl)acetic acid (BSAA) and 5,6-dichloroindole-3-acetic acid methyl ester (5,6-Cl2-IAA-Me) at different concentrations, respectively. Each chemical produced the maximum number of adventitious roots at a different concentration. Compared with IBA treatment, 5,6-Cl2-IAA-Me and BSAA treatments significantly increased root numbers on hypocotyl cuttings at lower concentration, particularly of 5,6-Cl2-IAA-Me treatment. Combinations of paclobutrazol (PB) with either 5,6-Cl2-IAA-Me or BSAA significantly stimulated the production of more adventitious roots than either chemical alone or combined. Capillary electrophoresis analysis have shown that the levels of IAA, IBA and BSAA in IBA plus PB or BSAA plus PB treatments were higher than those of IBA or BSAA alone. It was suggested that the cause of the synergistic effect of IBA (or BSAA) plus PB treatment might be due to increased endogenous auxin level. The activities of peroxidase and IAA oxidase in the rooting zone coincided with root development, indicating that the activities of these two enzymes were positively correlated to rooting. Peroxidase and IAA oxidase activity in all treatments started 24 h and 12 h after cutting, respectively. It is suggested that the major role of IAA oxidase differed from that of peroxidase in adventitious root formation.
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References
Bansal MP and Nanda KK (1981) IAA oxidase activity in relation to adventitious root formation on stem cuttings of some forest tree species. Experimentia 37: 1273–1274
Bhattacharya NC, Kaur NP and Nanda KK (1975) Transients in isoperoxidase during rooting etiolated stem segments of Populus nigra. Biochemie Physiologie Pflanzen 67: 159–164
Bryant SD and Lane FE (1979) Indole-3-acetic acid oxidase from peas. I. occurrence and distribution of peroxidative and nonperoxidative form. Plant Physiol 63: 696–699
Chibbar RN, Gurumurti and Nanda KK (1980) Effect of maleic hydrazide on peroxidase isoenzymes in relation to rooting hypocotyl cuttings of Phaseolus mungo. Biologia Plantarum 22: 1–6
Epstein E, Chen KH and Cohen JD (1989) Identification of indole-3-butyric acid as an endogenous constituents of maize kernels and leaves. Plant Growth Regul 8: 215–223
Epstein E and Ludwig-Muller J (1993) Indole-3-butyric acid in plants:occurrence, synthesis, metabolism and transport. Physiol Plant 88: 382–389
Ernstsen A and Sandberg G (1986) Identification of 4-chloroindole-3-acetic acid and indole-3-aldehyde in seeds of Pinus sylvestris. Plant Physiol 68: 511–518
Frenkel C and Hess CE (1974) Isozymic changes in relation to root initiation in mung bean. Can J Bot 52: 295–297
Gaspar T, Penel C, Castillo FJ and Greppin H (1985) A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiol Plant 64: 418–423
Gaspar T, Kevers C, Hausman JF, Berthon JY and Ripetti V (1992) Practical uses of peroxidase activity as predictive marker of rooting performance of micropropagated shoots. Agronomie 12: 757–765
Gaspar T (1995) Seleniated forms of indolyacetic acid - New powerful synthetic auxins. Acros Organics Acta 1(2): 65–66
Hartmann HT, Kester DE and Davies FT (1990) Plant Propagation: Principles and Practices, pp 246–247. Englwood Cliffs, NJ: Prentice Hall
Jarvis BC (1986) Endogenous control of adventitious rooting in non-woody cuttings. In Jackson MB (eds) New Root Formation Plants and Cuttings, pp 191–222. Dordrecht: Martinus Nijhoff Publishers
Katayama M and Futatsuya F (1990) Synthesis and biological activities of chlorinated indole auxins and antiauxins. Plant Cell Engineering 2(4): 487–502
Ludwig-Muller J, Sass S, Sutter EG, Wodner M and Epstein E (1993) Indole-3-butyric acid in Arabidopsis thaliana. 1. Identification and quantification. Plant Growth Regul 13: 179–187
Marumo S, Abe H, Hattori H and Munakata K (1968) Isolation of novel auxin, methyl 4-chloroindoleacetate, from immature seeds of Pisum sativum. Agric Biol Chem 32: 117–118
Moncousin C and Gaspar T (1983) Peroxidase as a marker for rooting improvement of Cynara scolymus L cultured in vitro. Biochem Physiol Pflanzen 178: 263–271
Nanda KK, Bhattachary NC and Kaur NP (1973) Effect of morphactin on peroxidases and its relationship to rooting hypocotyl cuttings of Impatiens balsamina. Plant Cell Physiol 14: 207–211
Nickell LG (1982) Plant Growth Regulators. Agricultural Uses, pp 4–5. New York: Springer-Verlag
Pan RC and Zhao ZJ (1994) Synergistic effects of plant growth retardants and IBA on the formation of adventitious roots in hypocotyl cuttings of mung bean. Plant Growth Regul 14: 15–19
Pan RC and Gui HY (1997) Physiological basis of the synergistic effects of IBA and triadimefon on rooting of mung bean hypocotyls. Plant Growth Regul 22: 7–11
Spector T (1978) Refinement of Coomassie blue method of protein quantitation. Anal Biochem 86: 142–146
Thorpe TA, Tran TVM and Gaspar T (1978) Isoperoxidases in epidermal layer of tobacco and changes during organ formation in vitro. Physiol Plant 44: 388–394
Tian XS and Pan RC (1998) Separation and quantitation of plant growth substances by capillary zone electrophoresis. J Chinese Chromatography (in press)
Wiesman Z and Riov J (1994) Interaction of paclobutrazol and indole-3-butyric acid in relation to rooting of mung bean (Vigna radiata) cuttings. Physiol Plant 92: 608–612
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Pan, R., Tian, X. Comparative effect of IBA, BSAA and 5,6-Cl2-IAA-Me on the rooting of hypocotyl in mung bean. Plant Growth Regulation 27, 91–98 (1999). https://doi.org/10.1023/A:1006154426941
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DOI: https://doi.org/10.1023/A:1006154426941