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Upstream and downstream sequence elements determine the specificity of the rice tungro bacilliform virus promoter and influence RNA production after transcription initiation

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Abstract

The contribution of sequences upstream and downstream of the transcription start site to the strength and specificity of the promoter of rice tungro bacilliform virus was analysed in transgenic rice plants. The promoter is strongly stimulated by downstream sequences which include an intron and is active in all vascular and epidermal cells. Expression in the vascular tissue requires a promoter element located between −100 and −164 to which protein(s) from rice nuclear extracts bind. Elimination of this region leads to specificity for the epidermis. Due to the presence of a polyadenylation signal in the intron, short-stop RNA is produced from the promoter in addition to full-length primary transcript and its spliced derivatives. The ratio between short-stop RNA and full-length or spliced RNA is determined by upstream promoter sequences, suggesting the assembly of RNA polymerase complexes with different processivity on this promoter.

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  1. Joachim Wünn

    Present address: Monsanto, European Crop Research Centre, Rue Laid Burniat 5, 1348, Louvain-la-Neuve, Belgium

Authors and Affiliations

  1. Institute of Plant Sciences, ETH Zürich, Universitätstrasse 2, 8092, Zürich, Switzerland

    Andreas Klöti, Cécile Henrich, Stéphane Bieri, Peter K. Burkhardt, Paola Lucca, Ingo Potrykus & Johannes Fütterer

  2. Friedrich Miescher Institute, P.O. Box 2543, 4002, Basel, Switzerland

    Xiaoyuan He, Gang Chen & Thomas Hohn

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Klöti, A., Henrich, C., Bieri, S. et al. Upstream and downstream sequence elements determine the specificity of the rice tungro bacilliform virus promoter and influence RNA production after transcription initiation. Plant Mol Biol 40, 249–266 (1999). https://doi.org/10.1023/A:1006119517262

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