Abstract
The concept of using sunlight for micropropagation systems is proposed as a way of reducing tissue culture costs. Shoot tips of Musa acuminata cultivar ‘Grande Naine’ were cultured in a non-controlled natural light environment at the IAEA Laboratories, Austria during summertime. Significantly more shoots were produced by plantlets cultivated in a sunlit room with photosynthetic photon flux densities (PPFD) fluctuating up to 570 μmol m-2 s-1, temperatures between 23 and 30 °C and photoperiods of 12 to 16-h, than by plantlets under artificial light in a growth chamber providing controlled conditions of a constant PPFD of 65 μmol m-2 s-1, temperatures ranging from 23 to 29 °C and a 16-h photoperiod. Highest multiplication rates were achieved in a greenhouse with PPFD reaching 860 μmol m-2 s-1 and temperatures of 18 – 43 °C, but browning of leaves and loss of turgor occurred. Nevertheless, rooted plantlets showed 100% survival during acclimatisation and normal development. Photoperiods of 12 – 16 h did not affect the multiplication rates.
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Kodym, A., Zapata-Arias, F.J. Natural light as an alternative light source for the in vitro culture of banana (Musa acuminata cv. ‘Grande Naine’). Plant Cell, Tissue and Organ Culture 55, 141–145 (1998). https://doi.org/10.1023/A:1006119114107
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DOI: https://doi.org/10.1023/A:1006119114107