Abstract
Callus and cell suspension cultures of Rauwolfia sellowii were established in Gamborg B5 medium supplemented with 1 mg l-1 2,4-dichlorophenoxyacetic acid, 0.2 mg l-1 kinetin and 30 g l-1 sucrose. The growth cycle of suspension cultures was completed in ca. 22 days and the maximum specific growth rate was 0.0098 h-1 with a doubling time of 71 h. The cultures accumulated the same major alkaloids as in the leaves of the parent plant, such as sellowiine, 19α,20α-epoxyakuammicine, vomilenine, picrinine and 12-demethoxytabernulosine. The alkaloid contents of leaves, callus and cell suspension cultures were quantitatively compared by HPLC.
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Rech, S., Batista, C., Schripsema, J. et al. Cell cultures of Rauwolfia sellowii: growth and alkaloid production. Plant Cell, Tissue and Organ Culture 54, 61–63 (1998). https://doi.org/10.1023/A:1006118220785
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DOI: https://doi.org/10.1023/A:1006118220785