Abstract
According to reported cytogenetic studies, there is a significant association between chromosomal aberrations and aggressiveness in meningiomas. With the method of restriction fragment length polymorphism analysis (RFLP), we examined tumor specific LOH on chromosome 17p and 22q in 30 cases of intracranial meningiomas. There were eight cases of meningiomas with aggressive characteristics, such as invasive meningioma, malignant meningioma, hemangiopericytic meningioma, and multiple meningiomas with central neurofibromatosis. Twenty-five of 30 cases (83%) were constitutionally heterozygous for at least one of the chromosome 22q DNA markers and sixteen of 25 informative cases (64%) displayed loss of heterozygosity (LOH). All of the 8 informative cases (100%) of meningiomas with aggressive characteristics, showed LOH on chromosome 22q whereas non-aggressive cases revealed LOH in eight of 17 informative cases (47%). At the loci on chromosome 17p, only two cases of malignant meningionas showed LOH. Our results suggest that the inactivations of putative tumor suppressor genes on chromosome 22q and 17p may correlate with aggressiveness and malignant transformation of meningiomas.
Similar content being viewed by others
References
Nakasu S, Hirano A, Llena JF, Shimura T, Handa J: Interface between the meningioma and the brain. Surg Neurol 32: 206–212, 1989
Russell DS, Rubinstein LJ: Pathology of tumours of the nervous system. (5th ed.) Edward Arnold, London, Melbourne, Auckland 1989, pp 449–505
Weller RO: Color atlas of neuropathology. Oxford University Press, New York 1984, pp 84–85
Zankle H, Zang KD: Correlation between clinical and cytogenetical data in 180 human meningiomas. Cancer Genet Cytogenet 1: 351–356, 1980
Zang KD: Cytological and cytogenetical studies on human meningioma. Cancer Genet Cytogenet 6: 249–274, 1982
Dumanski JP, Carlbom E, Collins VP, Nordenskjold M: Deletion mapping of a locus on human chromosome 22 involved in the oncogenesis of meningioma. Proc Natl Acad Sci USA 84: 9275–9279, 1987
Dumanski JP, Roulcau GA, Nordenskjold M, Collins VP: Molecular genetic analysis of chromosome 22 in 81 cases of meningioma. Cancer Res 50: 5863–5867, 1990
Meese E, Blin N, Zang KD: Loss of heterozygosity and origin of meningioma. Hum Genet 77: 349–351, 1987
Seizinger BR, Monte SDL, Atkins L, Gusella JF, Martuza RL: Molecular genetic approach to human meningioma: loss of genes on chromosome 22. Proc Natl Acad Sci USA 84: 5419–5423, 1987
Seizinger BR, Rouleau G, Ozelius LJ, Lane AH, George-Hyslop P, Huson S, Gusella JF, Martuza RL: Common pathogenetic mechanism for three tumor types in bilateral acoustic neurofibromatosis. Science 236: 317–319, 1987
Fults D, Brockmeyer D, Tullous MW, Pedone CA, Cawthon RM: p53 mutation and loss of heterozygosity on chromosomes 17 and 10 during human astrocytoma progression. Cancer Res 52: 674–679, 1992
James WK: Clonal genomic alterations in glioma malignancy stages. Cancer Res 48: 5546–5551, 1988
Raffel C, Gilles FE, Weinberg KI: Reduction to homozygosity and gene amplification in central nervous system primitive neuroectodermal tumors of childhood. Cancer Res 50: 587–591, 1990
Cavenee WK, Dryja TP, Phillips RA, Benedict WF, Godbout R, Gallic BL, Murphrce AL, Strong LC, White R: Expression of recessive alleles by chromosomal mechanisms in retinoblastoma. Nature 305: 779–784, 1983
Feinberg A, Vogelstein B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6, 1983
Nigro JM, Baker SJ, Preisinger AC, Jessup JM, Hostetter R, Cleary K, Binger SH, Davidson N, Baylin S, Devilee P, Glover T, Collins FS, Weston A, Modari R, Harris CC, Vogelstein B: Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705–708, 1989
Al Saadi A, Latimer F, Madercic M, Robbins T: Cytogenetic studies of human brain tumors and their clinical significance. II. Meningioma. Cancer Genet Cytogenet 26: 127–141, 1982
Sanson M, Richard S, Delattre O, Poliwka M, Mikol J, Philippon J, Thomas G: Allelic loss on chromosome 22 correlates with histopathological predictors of recurrence of meningiomas. Int J Cancer 50: 392–394, 1992
Christiansen H, Lampert F: Tumor karyotype discriminates between good and bad prognostic outcome in neuroblastoma. Brit J Cancer 57: 121–126, 1988
Fong CT, Dracopoli NC, White PS, Merrill PT, Griffith RC, Housman DE, Brodeur GM: Loss of heterozygosity for the short arm of chromosome 1 in human neuroblastomas: correlation with N-myc amplification. Proc Natl Acad Sci USA 68: 3753–3757, 1989
Fujimoto M, Fults DW, Thomas GA, Nakamura Y, Heilbrun MP, White R, Story JL, Naylor SL, Kagan-Hallet KS, Sheridan PJ: Loss of heterozygosity chromosome 10 in human glioblastoma multiforme. Genomics 4: 210–214, 1989
Cogen PH, Daneshvar L, Metzger AK, Edwards MSB: Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279–285, 1990
Mashiyama S, Murakami Y, Yoshimoto T, Sekiya T, Hayashi K: Detection of p53 gene mutations in human brain tumors by single-strand conformation polymorphism analysis of polymerase chain reaction products. Oncogene 6: 1313–1318, 1991
Pykett MJ, Landers J, George DL: Expression parterns of the p53 tumor suppressor gene and the mdm proto-oncogene in human meningiomas. J Neurooncol 32(1): 39–44, 1997
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, JH., Lee, SH., Rhee, CH. et al. Loss of heterozygosity on chromosome 22q and 17p correlates with aggressiveness of meningiomas. J Neurooncol 40, 101–106 (1998). https://doi.org/10.1023/A:1006110812240
Issue Date:
DOI: https://doi.org/10.1023/A:1006110812240