Abstract
Multiple shoots were produced from nodal explants, of 30-day-old in vitro grown seedlings and from pretreated 3- and 9-month-old greenhouse grown Acacia mearnsii plants, respectively. Explants were sterilized using 0.1% and 0.2% HgCl2 for 15 min for 3- and 9-month-old explants, respectively. Nodal explants were induced to form multiple shoots when placed on MS medium supplemented with 2.0 mg l −1 benzyladenine. Rooting of these shoots was achieved on MS medium supplemented with 1.0 mg l −1 indole-3-butyric acid. Plantlets were acclimatized in transparent plastic containers under greenhouse conditions with a 90% success rate.
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Beck, S.L., Dunlop, R. & van Staden, J. Micropropagation of Acacia mearnsii from ex vitro material. Plant Growth Regulation 26, 143–148 (1998). https://doi.org/10.1023/A:1006109603715
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DOI: https://doi.org/10.1023/A:1006109603715