Abstract
Cold treatment of surface sterilized explant material of hardwood trees at 4°C for two or four days reduced or eradicated internal contamination with Bacillus subtilis. This procedure provides a cheap, rapid and non-toxic technique to introduce material from adult trees, growing out of doors, into culture.
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Kowalski, B., van Staden, J. Cold treatment, as part of the process, improves explant decontamination. Plant Growth Regulation 26, 203–205 (1998). https://doi.org/10.1023/A:1006104525353
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DOI: https://doi.org/10.1023/A:1006104525353