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Characterization of three novel members of the Arabidopsis SHAGGY-related protein kinase (ASK) multigene family

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Abstract

In this paper we report the characterization of three novel members of the Arabidopsis shaggy-related protein kinase (ASK) multigene family, named ASKdzeta (ASKζ), ASKetha (ASKη) and ASKiota (ASKι). The proteins encoded by the ASK genes share a highly conserved catalytic protein kinase domain and show about 70% identity to SHAGGY (SGG) and glycogen synthase kinase-3 (GSK-3) from Drosophila and rat respectively. SGG is an ubiquitous intracellular component of the wingless signalling pathway that establishes cell fate and/or pattern formation in Drosophila. At least ten different ASK genes are expected to be present per haploid genome of A. thaliana. Different amino- and carboxy-terminal extensions distinguish different ASK family members. Five ASK gene sequences were analysed and shown to be present as single-copy genes in the Arabidopsis genome. A comparison based on the highly conserved catalytic domain sequences of all known sequences of the GSK-3 subfamily of protein kinases demonstrated a clear distinction between the plant and the animal kinases. Furthermore, we established the presence of at least three distinct groups of plant homologues of SGG/GSK-3. These different groups probably reflect biochemical and/or biological properties of these kinases. The differential expression patterns of five ASK genes were accessed by northern and in situ hybridization experiments using gene-specific probes. While ASKζ is expressed in the whole embryo during its development, ASKη expression is limited to the suspensor cells. No signal was detected for ASKα, ASKγ and ASKι in developing embryos.

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Authors and Affiliations

  1. Institut de Biotechnologie des Plantes ERS/CNRS 569, Université de Paris-Sud, Bâtiment, 630, 91405 , Orsay Cedex, France

    Marcelo Carnier Dornelas & Martin Kreis

  2. Laboratory of Plant Cytology and Morphology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD, Wageningen, Netherlands

    Peter Wittich, Iris von Recklinghausen & André van Lammeren

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  1. Marcelo Carnier Dornelas
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Dornelas, M.C., Wittich, P., von Recklinghausen, I. et al. Characterization of three novel members of the Arabidopsis SHAGGY-related protein kinase (ASK) multigene family. Plant Mol Biol 39, 137–147 (1999). https://doi.org/10.1023/A:1006102812280

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