The role of 5-aminolevulinic acid (5-ALA) as a precursor of chlorophyll and a herbicide is well documented. 5-ALA as a Plant Growth substance is also proven in recent times. In the present report, to elucidate the physiological effects of 5-ALA, the compound was used in in vitro studies using MS medium supplemented with 5-ALA at 2, 5 and 10 mg l-1. Leaf and cotyledonary node were used as the explants. In vitro studies confirmed the hormonal role of 5-ALA by striking proliferation of callus paripassu induction of rooting and shooting with a profound effect of the former than the latter. Thus, 5-ALA has the dual properties of auxin and cytokinin in the induction of callusing and rhizogenesis, and shooting respectively.
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Angeloni PN, Rey HY and Mroginski LA (1992) Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum. Plant Cell Rep 11: 519-521
Anju Gulati and Jaiwal PK (1992) In vitro induction of multiple shoots and plant regeneration from shoot tips of mungbean (Vigna radiata (L.) Wilczek). Plant Cell, Tissue and Organ Culture 29: 199-205
Bandara PMS and Tanino KK (1995) Paclobutrazol enhances, minituber production in Norland potatoes. J Plant Growth Regul 14: 151-155
Bindu Roy C and Vivekanadan M (1988) A new role for 5-aminolevulinic acid as a plant growth regulator. Appl Biochem and Biotech (in press)
Bindu Roy C and Vivekanandan M (1998) Role of 5-aminolevulinic acid in improving biomass and yield in Vigna catjung, V. mungo and V. radiata. Biol Plant (in press)
Hotta Y, Tanaka T, Takaola H, Takeuchi Y and Konnai M (1997) Promotive effects of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regul 22: 109-114
Kerns HR, Barwale UB, Meyer Jr MM and Widholm JM (1986) Correlation of cotyledonary node shoot proliferation and somatic embryoid development in suspension cultures of soybean (Glycine max L. Merr). Plant Cell Rep 5: 140-143
Kuti JO and Jarvis BB (1992) Growth hormone-like activities of macrocyclic trichothecenes in in vitro callus induction and growth of four Baccharis species. J Plant Growth Regul 11: 149-154
Mohanty BD, Dutta Gupta S and Ghosh PD (1985) Callus initiation and plant regeneration in ragi (Elucine coracana Gaertn.). Plant Cell, Tissue and Organ Culture 5: 147-150
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473-497
Polowick PL and Sawhney VK (1991) In vitro floral development of oilseed rape (Brassica napus L.): The effects of pH and plant growth regulators. J Exp Botany 42(245): 1583- 1588
Ravnikar M, Zel J, Plaper I and Spacapan A (1993) Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. Plant Growth Regul 12: 73-77
Rebeiz CA, Montazer-Zouhoor A, Hopen HJ and Wu SM (1984) Photodynamic herbicides: Concept and phenomenology. Enzymol Microb Technol 6: 390-401
Rebeiz CA, Reddy KN, Nandhihalli UB and Velu J (1990) Tetrapyrrole - dependent photodynamic herbicides. Photochem and Photobiol 52(6): 1099-1117
Sasaki K, Marquez FJ, Nishio N and Nagai S (1995) Promotive effect of 5-aminolevulinic acid on the growth and photosynthesis of Spirulina platensis. J Ferment Bioeng 79: 453- 457
Saxena C, Palai SK, Samantary S, Rout GR and Das P (1997) Plant regeneration from callus cultures of Psoralea corylifolia Linn. Plant Growth Regul 22: 13-17
Smith MK and Drew RA (1990) Current applications of tissue culture in plant propagation and improvement. Aust J Plant Physiol 17: 267-289
Reinbothe S and Reinbothe C (1996) Regulation of chlorophyll biosynthesis in angiosperms. Plant Physiol 111: 1-7
Tanaka T, Takahashi K, Hotta Y, Takeuchi Y and Konnai M (1992) Promotive effects of 5-aminolevulinic acid on yield of several crops. In: 19th PGR Society of America, San Francisco, pp 3-9
Van der Kriekan, Croes AF, Hermans M and Wullems GJ (1991) Induction of flower bud formation in vitro by Dihydrozeatin. Plant Growth Regul 10: 79-83
Leuba V and Le Tourneau D (1990) Auxin activity of phenyl acetic acid in tissue culture. J Plant Growth Regul 9: 71-76
Magnus V, Nigovic B, Hangertar RP and Good NE (1992) (Indol-3-yl acetyl) Amino acids as a source of auxin in plant tissue culture. J Plant Growth Regul 11: 19-28
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Bindu, R.C., Vivekanandan, M. Hormonal activities of 5-aminolevulinic acid in callus induction and micropropagation. Plant Growth Regulation 26, 15–18 (1998). https://doi.org/10.1023/A:1006098005335