Veterinary Research Communications

, Volume 22, Issue 4, pp 249–255 | Cite as

Novel p53 tumour suppressor mutations in cases of spindle cell sarcoma, pleomorphic sarcoma and fibrosarcoma in cats

  • B. Mayr
  • M. Reifinger
  • K. Alton
  • G. Schaffner


Twenty feline neoplasms were sequenced in the region from exons 5 to 8 for the presence of tumour suppressor gene p53 mutations. In a spindle cell sarcoma of the bladder, a missense mutation (codon 164 AAG→GAG, lysine→glutamic acid) in exon 5 was detected. In a pleomorphic sarcoma, a 23 bp deletion involving the splicing junction between intron 5 and exon 6 was observed. In a fibrosarcoma, a 6 bp deletion of p53 covering 2 bp of exon 7 and 4 bp of intron 7, including the splicing junction, was found. The study demonstrates three new p53 mutations in different types of sarcomas in cats.

cat mutation p53 gene sarcoma tumour 


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  1. Breau, R.L. and Clayman, G.L., 1996. Gene therapy for head and neck cancer. Current Opinion in Oncology, 8, 227-231Google Scholar
  2. De Vries, E.M.G., Ricke, D.O., De Vries, T.N., Hartmann, A., Blaszyk, H., Liao, D., Soussi, T., Kovach, J.S. and Sommer, S.S., 1996. Database of mutations in the p53 and APC tumor suppressor genes designed to facilitate molecular epidemiological analyses. Human Mutation, 7, 202-213Google Scholar
  3. Hollstein, M., Shomer, B., Greenblatt, M., Soussi, T., Hovig, E., Montesano, R. and Harris, C.C., 1996. Somatic point mutations in the p53 gene of human tumors and cell lines: updated compilation. Nucleic Acids Research, 24, 141-146Google Scholar
  4. Levine, A.J., Momand, J. and Finlay, C.A., 1991. The p53 tumour suppressor gene. Nature, 351, 453-456Google Scholar
  5. Mayr, B., Heczko, U., Schellander, K., Schleger, W. and Reifinger, M., 1993. Sequence of an exon of the feline p53 gene - mutation in a lymphosarcoma. British Veterinary Journal, 149, 387-390Google Scholar
  6. Mayr, B., Schaffner, G., Kurzbauer, R., Schneider, A., Reifinger, M. and Loupal, G., 1995a. Mutations in tumour suppressor gene p53 in two feline fibrosarcomas. British Veterinary Journal, 151, 707-713Google Scholar
  7. Mayr, B., Schaffner, G., Kurzbauer, R., Reifinger, M. and Schellander, K., 1995b. Sequence of an exon of tumour suppressor p53 gene - a comparative study in domestic animals. Mutation in a feline solid mammary carcinoma. British Veterinary Journal, 151, 325-329Google Scholar
  8. Mayr, B., Reifinger, M. and Loupal, G., 1998. Polymorphisms in feline tumour suppressor gene p53. Mutations in an osteosarcoma and a mammary carcinoma. Veterinary Journal, 155, 103-106Google Scholar
  9. Muüllenbach, J.P., Lagoda, L. and Welter, C., 1989. An efficient salt-chloroform extraction of DNA blood and tissues. Trends in Genetics, 5, 391Google Scholar
  10. Okuda, M., Umeda, A., Sakai, T., Ohashi, T., Momoi, Y., Youn, H.Y., Watari, T., Goitsuka, R., Tsujimoto, H. and Hasegawa, A., 1994. Cloning of feline p53 tumor-suppressor gene and its aberration in hematopoietic tumor. International Journal of Cancer, 58, 602-607Google Scholar
  11. Roth, J.A., Nguyen, D., Lawrence, D.D., Kemp, B.L., Carrasco, C.H., Ferson, D.Z., Hong, W.K., Komaki, R., Lee, J.J., Nesbitt, J.C., Pisters, K.M., Putnam, J.B., Schea, R., Shin, D.M., Walsh, G.L., Dolormente, M.M., Han, C.I., Martin, F.D., Yen, N., Xu, K., Stephens, L.C., McDonell, T.J., Mukhopadhyay, T. and Cai, D., 1996. Retrovirus-mediated wild-type p53 gene transfer to tumors of patients with lung cancer (see comments). Nature Medicine, 2, 974-975Google Scholar
  12. Soussi, T., 1995. The p53 tumour suppressor gene: from molecular biology to clinical investigation. In: J.K. Cowell (ed.), Molecular Genetics of Cancer, (Bios Scientific Publishers), 135-178Google Scholar
  13. Soussi, T., De Fromentel, C. and May, P., 1990. Structural aspects of the p53 protein in relation to gene evolution. Oncogene, 5, 945-952Google Scholar
  14. Vogelstein, B. and Kinzler, K.W., 1992. p53 function and disfunction. Cell, 70, 523-526Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • B. Mayr
    • 1
  • M. Reifinger
    • 2
  • K. Alton
    • 2
  • G. Schaffner
    • 3
  1. 1.Institute for Animal Breeding and Genetics, Veterinary UniversityViennaAustria
  2. 2.Institute for Pathology and Forensic Veterinary Medicine, Veterinary UniversityViennaAustria
  3. 3.Research Institute of Molecular Pathology, Dr Bohr-Gasse 7ViennaAustria

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