Abstract
The behavior of the autonomous maize transposable element En/Spm of maize was studied in Arabidopsis. Transgenic Arabidopsis plants carrying En-1 elements were propagated for 12 generations using a single seed descent procedure. The distribution and activity of the En-1 element was monitored using Southern DNA hybridisations in generations 1, 6 and 12. In the first generation the highest number of En-1 insertions per line was 7, which increased to 20 in generation 12. The average number of En-1 insertions increased only slightly in the population, due to a gradual accumulation of segregants that lost the transposable element. During the development of the En-1 mutagenised population the element remained active even in the high-copy lines. In situ hybridisation demonstrated that multiple En-1 insertions were distributed over all Arabidopsis chromosomes. From the initial En-1 mutagenised populations many unstable gene mutations were recovered, indicating that En-1 can be used as a efficient tool for gene tagging in Arabidopsis.
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References
Aarts GM, Dirks WJ, Stiekema WJ, Pereira A: Transposable element tagging of a male sterility gene in Arabidopsis. Nature 363: 715–717 (1993).
Aarts GM, Keijzer CJ, Stiekema W J, Pereira A: Molecular characterisation of the CER1 gene of Arabidopsis thaliana involved in epicuticular wax biosynthesis and pollen fertility. Plant Cell 7: 2115–2127 (1995).
Berg DE, Howe MM (eds): Mobile DNA. American Society of Microbiology, Washington, DC (1989).
Bhatt AJ, Page T, Lawson EJR, Lister C, Dean C:Use of Ac as an insertional mutagen in Arabidopsis. Plant J 9: 935–945 (1996).
Bonas U, Sommer H, Harrison BJ, Saedler H: The transposable element Tam1 of Antirrhinum majus is 17 kb long. Mol Gen Genet 194: 138–143 (1984).
Bradley D, Carpenter R, Sommer H, Hartley N, Coen, E: Complementary floral homeotic phenotypes result from opposite orientations of a transposable element at the PLENA locus of Antirrhinum majus. Cell 72: 85–95 (1993).
Campbell BR, Song Y, Posch TE, Cullis CA, Town CD: Sequence and organization of 5S ribosomal RNA-encoding genes of Arabidopsis thaliana. Gene 112: 225–228 (1992).
Cardon GH, Frey M, Saedler H, Gierl A: Mobility of the maize transposable element En/Spm in Arabidopsis thaliana. Plant J 3: 773–784 (1993).
Cocciolone SM, Cone K: Pl-Bh, an anthocyanin regulatory gene of maize that leads to variegated pigmentation. Genetics 135: 575–588 (1993).
Cuypers H, Sudhansu D, Peterson P, Saedler H, Gierl: The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays. EMBO J 7: 2953–2960 (1988).
Dash S, Peterson PA: Frequent loss of the En transposable element after excision and its relation to chromosome replication in maize (Zea mays L.). Genetics 136: 653–671 (1994).
Edwards K, Johnstone C, Thompson C: A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl Acids Res 19: 1349 (1991).
Federoff NV: Maize transposable elements. In: Berg DE, Howe MM (eds) Mobile DNA, pp. 374–411. American Society of Microbiology, Washington DC (1989).
Feldmann KA: T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum. Plant J 1: 71–82 (1991).
Fransz PF, Stam M, Montijn B, ten Hoopen R, Wiegant J, Kooter JM, Oud O, Nanninga N: Detection of single-copy genes and chromosome rearrangements in Petunia hybrida by fluorescence in situ hybridisation. Plant J 9: 767–774 (1996).
Frey M, Reinecke J, Grant S, Saedler H, Gierl A: Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins. EMBO J 9: 4037–4044 (1990).
Greenblatt IM, Brink RA: Twin mutations in medium variegated pericarp maize. Genetics 47: 489–501 (1962).
Hake S, Vollbrecht E, Freeling M: Cloning knotted, the dominant morphological mutant in maize using Ds2 as a transposable element tag. EMBO J 8: 15–22 (1989).
Koncz C, Rédei GP: Genetic studies with Arabidopsis: a historical view. In: Meyerowitz EM, Somerville CR (eds), pp. 223–253. Arabidopsis, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1994).
Kunze R, Saedler H, Lönnig WE: Plant transposable elements. Adv Bot Res 27: 331–469 (1997).
McClintock B: Mutations in maize and chromosomal abberations in Neurospora. Carnegie Inst Wash Yearb 53: 254–260 (1954).
McClintock B: Mutable loci in maize. Carnegie Inst Wash Yearb 47: 155–169 (1948).
Nevers P, Shepherd N, Saedler, H: Transposable elements. Adv Bot Res 12: 102–203 (1986).
Pereira A, Saedler H: Transpositional behaviour of the maize En/Spm element in transgenic tobacco. EMBO J. 8: 1315–1321 (1989).
Pereira A, Cuypers H, Gierl A, Schwartz-Sommer Zs, Saedler H: Molecular analysis of the En/Spm transposable element system of Zea mays. EMBO J 5: 835–841(1986).
Pereira A, Schwarz-Sommer Zs, Gierl A., Peterson PA, Saedler H: Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays. EMBO J. 4: 17–23 (1985).
Peterson PA: Amutable pale locus in maize. Genetics 45: 115–133 (1953).
Ross KJ, Fransz P, Jones G: A light microscope atlas of meiosis in Arabidopsis thaliana. Chromos Res 4: 507–516 (1996).
Schwarz-Sommer Zs, Gierl A, Klösgen RB, Wienand U, Peterson PA, Saedler H: The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx-m8 allele of Zea mays. EMBO J 3: 2439–2443 (1984).
Schwarz-Sommer Zs, Gierl A, Cuypers H, Peterson PA, Saedler H: Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J 4: 591–597 (1985).
Tsay Y-F, Frank MJ, Page T, Dean C, Crawford NM: Identification of a mobile endogenous transposon in Arabidopsis thaliana. Science 260: 342–344 (1993).
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Wisman, E., Cardon, G.H., Fransz, P. et al. The behaviour of the autonomous maize transposable element En/Spm in Arabidopsis thaliana allows efficient mutagenesis. Plant Mol Biol 37, 989–999 (1998). https://doi.org/10.1023/A:1006082009151
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DOI: https://doi.org/10.1023/A:1006082009151