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Effects of cytokinins and auxins on cassava shoot organogenesis and somatic embryogenesis from somatic embryo explants

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Abstract

Somatic embryos of cassava (Manihot esculenta Crantz) cultivar ‘Nanzhi 188’ were isolated and cut into fragments to be cultured on media with various cytokinins and auxins. Cytokinin induced of cassava shoot organogenesis, while auxin stimulated somatic embryogenesis. The effectiveness on organogenesis was different based on different cytokinins and in combination with auxins. Benzyladenine and thidiazuron stimulated more shoot organogenesis than kinetin and N-isopentenyladenine.

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References

  • George L & Eapen S (1994) Organogenesis and embryogenesis from diverse explants in pigeonpea (Cajanus cajan L.). Plant Cell Rep. 13(7): 417–420

    Article  CAS  Google Scholar 

  • Guo JY & Liu YQ (1995) Rapid propagation of cassava by tissue culture and its application in rural districts in China. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN), Vol I (pp 183–189). Bogor. Indonesia, 22–26 August 1994. CIAT, Cali, Colombia

    Google Scholar 

  • Konan NK, Sangwan RS & Sangwan BC (1994) Somatic embryogenesis from cultured mature cotyledons of cassava (Manihot esculenta Crantz). Plant Cell Tiss Org. Cult. 37: 91–102

    Article  Google Scholar 

  • Li HQ, Huang YW, Liang CY & Guo JY (1995) Improvement of plant regeneration from secondary somatic embryos of cassava. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN), Vol. I, (pp 289–302). Bogor, Indonesia, 22–26 August 1994. CIAT, Cali, Colombia

    Google Scholar 

  • Li HQ, Sautter C, Potrykus I & Puonti-Kaerlas J (1996) Genetic transformation of cassava (Manihot esculenta Crantz). Nature Biotechnology 14: 736–740

    Article  PubMed  CAS  Google Scholar 

  • Mathews H, Schopke C, Carcamo R, Fauquet C & Beachy RN (1993) Improvement of somatic embryogenesis and plant recovery in cassava. Plant Cell Rep. 12: 328–333

    Article  Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Raemakers CJJM, Schavemaker CM, Jacobsen E & Visser RGF (1993) Improvements of cyclic somatic embryogenesis of cassava (Manihot esculenta Crantz). Plant Cell Rep. 12: 226–229

    Article  Google Scholar 

  • Raemakers CJJM, Sofiari B, Kanju B, Jacobsen E & Visser RGF (1995) NAA-induced somatic embryogenesis in cassava. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN).Vol I, (pp 355–363). Bogor, Indonesia, 22–26 August 1994. CIAT, Cali, Colombia

    Google Scholar 

  • Schopke C, Chavarriaga P, Fauquet C & Beachy RN (1993) Cassava tissue culture and transformation: improvement of culture media and the effect of different antibiotics on cassava. In: Proceeding of First International Scientific Meeting of the Cassava Biotechnology Network (CBN), (pp 140–145). 25–28 August, 1992. Cartagena, Colombia

  • Stamp JA & Henshaw GG (1982) Somatic embryogenesis in cassava. Z Pflanzenphysiol. 105: 183–187

    Google Scholar 

  • Stamp JA & Henshaw GO (1986) Adventitious regeneration in cassava. In: Withers LA & Alderson PG (ed) Plant Tissue Culture and its Agronomical Applications (pp 149–157). Butterworths, London

    Google Scholar 

  • Szabados L, Hoyos R & Roca W (1987) In vitro somatic embryogenesis and plant regeneration of cassava. Plant Cell Rep. 6: 248–251

    Article  CAS  Google Scholar 

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Guohua, M. Effects of cytokinins and auxins on cassava shoot organogenesis and somatic embryogenesis from somatic embryo explants. Plant Cell, Tissue and Organ Culture 54, 1–7 (1998). https://doi.org/10.1023/A:1006065120629

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  • DOI: https://doi.org/10.1023/A:1006065120629

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