Abstract
Somatic embryos of cassava (Manihot esculenta Crantz) cultivar ‘Nanzhi 188’ were isolated and cut into fragments to be cultured on media with various cytokinins and auxins. Cytokinin induced of cassava shoot organogenesis, while auxin stimulated somatic embryogenesis. The effectiveness on organogenesis was different based on different cytokinins and in combination with auxins. Benzyladenine and thidiazuron stimulated more shoot organogenesis than kinetin and N-isopentenyladenine.
Similar content being viewed by others
References
George L & Eapen S (1994) Organogenesis and embryogenesis from diverse explants in pigeonpea (Cajanus cajan L.). Plant Cell Rep. 13(7): 417–420
Guo JY & Liu YQ (1995) Rapid propagation of cassava by tissue culture and its application in rural districts in China. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN), Vol I (pp 183–189). Bogor. Indonesia, 22–26 August 1994. CIAT, Cali, Colombia
Konan NK, Sangwan RS & Sangwan BC (1994) Somatic embryogenesis from cultured mature cotyledons of cassava (Manihot esculenta Crantz). Plant Cell Tiss Org. Cult. 37: 91–102
Li HQ, Huang YW, Liang CY & Guo JY (1995) Improvement of plant regeneration from secondary somatic embryos of cassava. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN), Vol. I, (pp 289–302). Bogor, Indonesia, 22–26 August 1994. CIAT, Cali, Colombia
Li HQ, Sautter C, Potrykus I & Puonti-Kaerlas J (1996) Genetic transformation of cassava (Manihot esculenta Crantz). Nature Biotechnology 14: 736–740
Mathews H, Schopke C, Carcamo R, Fauquet C & Beachy RN (1993) Improvement of somatic embryogenesis and plant recovery in cassava. Plant Cell Rep. 12: 328–333
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497
Raemakers CJJM, Schavemaker CM, Jacobsen E & Visser RGF (1993) Improvements of cyclic somatic embryogenesis of cassava (Manihot esculenta Crantz). Plant Cell Rep. 12: 226–229
Raemakers CJJM, Sofiari B, Kanju B, Jacobsen E & Visser RGF (1995) NAA-induced somatic embryogenesis in cassava. In: Proceeding of Second International Scientific Meeting of the Cassava Biotechnology Network (CBN).Vol I, (pp 355–363). Bogor, Indonesia, 22–26 August 1994. CIAT, Cali, Colombia
Schopke C, Chavarriaga P, Fauquet C & Beachy RN (1993) Cassava tissue culture and transformation: improvement of culture media and the effect of different antibiotics on cassava. In: Proceeding of First International Scientific Meeting of the Cassava Biotechnology Network (CBN), (pp 140–145). 25–28 August, 1992. Cartagena, Colombia
Stamp JA & Henshaw GG (1982) Somatic embryogenesis in cassava. Z Pflanzenphysiol. 105: 183–187
Stamp JA & Henshaw GO (1986) Adventitious regeneration in cassava. In: Withers LA & Alderson PG (ed) Plant Tissue Culture and its Agronomical Applications (pp 149–157). Butterworths, London
Szabados L, Hoyos R & Roca W (1987) In vitro somatic embryogenesis and plant regeneration of cassava. Plant Cell Rep. 6: 248–251
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guohua, M. Effects of cytokinins and auxins on cassava shoot organogenesis and somatic embryogenesis from somatic embryo explants. Plant Cell, Tissue and Organ Culture 54, 1–7 (1998). https://doi.org/10.1023/A:1006065120629
Issue Date:
DOI: https://doi.org/10.1023/A:1006065120629