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A ca. 40 kDa maize (Zea mays L.) embryo dehydrin is encoded by the dhn2 locus on chromosome 9

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Abstract

Dehydrins (LEA D11 proteins) are the products of multigene families in a number of higher plants [5]. To date, however, only one dehydrin locus, dhn1 (a major embryo and drought-induced protein of ca. 18 kDa) has been placed on chromosome 6L of the genetic linkage map of maize. The presence of a larger, ca. 40 kDa embryo protein that is also specifically detected by anti-dehydrin antibodies had been observed in some maize inbreds, including B73, suggesting that other dhn loci may exist. The ca. 22 kDa and ca. 40 kDa immunopositive proteins were purified from B73 and their amino acid compositions determined. The two proteins' amino acid compositions are typical of dehydrins, yet they differ from each other, indicating that they are distinct dhn gene products. Different size alleles for both proteins, or presence/absence in the case of the ca. 40 kDa protein, were evident from comparisons of embryo proteins of various maize inbreds. Analysis of segregating F2 progeny derived from self-pollination of F1 hybrids from four crosses (B73 × OH43, Mo17 × A632, AHO × A632, Latente × A632) revealed that alleles of the two genes assort independently. Map positions of the two dhn loci were then determined using two maize recombinant inbred line (RIL) mapping populations. The predicted map position of the gene controlling production of the ca. 22 kDa protein confirmed that this protein is the product of the dhn1 gene. The gene encoding the ca. 40 kDa dehydrin-like protein maps to a new locus on chromosome 9S near wx1, which we have named dhn2.

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References

  1. Asghar R, Fenton RD, DeMason DA, Close TJ: Nuclear and cytoplasmic localization of maize embryo and aleurone dehydrin. Protoplasma 177: 87–94 (1994).

    Google Scholar 

  2. Burr B, Burr F: Recombinant inbreds for molecular mapping in maize: theoretical and practical consideration. Trends Genet 7: 55–60 (1991).

    PubMed  Google Scholar 

  3. Campbell SA, Close TJ: Dehydrins: genes, proteins, and associations with phenotypic traits. New Phytol 137: 61–74 (1997).

    Google Scholar 

  4. Ceccardi TL, Meyer NC, Close TJ: Purification of a maize dehydrin. Protein Expr Purif 5: 266–269 (1994).

    PubMed  Google Scholar 

  5. Close TJ: Dehydrins: a commonality in the response of plants to dehydration and low temperature. Physiol Plant 100: 291–296 (1997).

    Google Scholar 

  6. Close TJ: Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant 97: 795–803 (1996).

    Google Scholar 

  7. Close TJ, Fenton RD, Moonan F: A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Mol Biol 23: 279–286 (1993).

    PubMed  Google Scholar 

  8. Close TJ, Kortt A, Chandler PM: A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13: 95–108 (1989).

    PubMed  Google Scholar 

  9. Dure L III: Structural motifs in LEA proteins of higher plants. In: Close TJ, Bray EA (eds) Response of Plants to Cellular Dehydration during Environmental Stress, pp. 91–103. American Society of Plant Physiologists, Rockville, TN (1993).

    Google Scholar 

  10. Harlow E, Lane D: Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1988).

    Google Scholar 

  11. Ismail AM, Hall AE, Close TJ: Chilling tolerance during emergence of cowpea associated with a dehydrin and slow electrolyte leakage. Crop Sci 37: 1270–1277 (1997).

    Google Scholar 

  12. Mao Z, Paiva R, Kriz AL, Juvik JA: Dehydrin gene expression in normal and viviparous embryos of Zea mays during seed development and germination. Plant Physiol Biochem 33: 649–653 (1995).

    Google Scholar 

  13. Moore G: Cereal genome evolution: pastoral pursuits with ‘Lego’ genomes. Curr Opin Genet Devel 5: 717–724 (1995).

    Google Scholar 

  14. Moore G, Foote T, Helentjaris T, Devos K, Kurata N, Gale M: Was there a single ancestral cereal chromosome? Trends Genet 11(3): 81–82 (1995).

    PubMed  Google Scholar 

  15. Plana M, Itarte E, Eritja R, Goday A, Pages M, Martinez MC: Phosphorylation of maize Rab17 protein by casein kinase 2. J Biol Chem 266: 22510–22514 (1991).

    PubMed  Google Scholar 

  16. Vertucci CW, Farrant JM: Acquisition and loss of dessication tolerance. In: Kigle J, Galili G (eds) Seed Development and Germination, pp. 237–271. Marcel Dekker, New York (1995).

    Google Scholar 

  17. Vilardell J, Goday A, Freire MA, Torrent M, Martinez MC, Torne JM, Pages M: Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize. Plant Mol Biol 14: 423–432 (1990).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521-0124, USA

    Scott A. Campbell, Donna E. Crone, Toni L. Ceccardi & Timothy J. Close

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  1. Scott A. Campbell
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  2. Donna E. Crone
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  3. Toni L. Ceccardi
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  4. Timothy J. Close
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Campbell, S.A., Crone, D.E., Ceccardi, T.L. et al. A ca. 40 kDa maize (Zea mays L.) embryo dehydrin is encoded by the dhn2 locus on chromosome 9. Plant Mol Biol 38, 417–423 (1998). https://doi.org/10.1023/A:1006037308167

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  • DOI: https://doi.org/10.1023/A:1006037308167