Abstract
The photoacoustic response of the photosynthetic apparatus to a short light pulse consists of three components: heat evolution, O2 evolution and CO2 uptake. Recent attempts of deconvoluting the individual components by curve-fitting by means of model curves [Kolbowski et al. (1990) Photosynth Res 25: 309–316] suffered from the fact that the model curve for CO2 uptake changed its curve shape with CO2 concentration. Here, it is shown that good fits can be obtained if a stretching factor is incorporated into the fitting routine which adjusts the shape of the uptake model curve. The relationship between CO2 uptake und H+ transport across the thylakoid membrane was investigated by experiments in different CO2 concentrations from 0 to 7%. It was found that under limiting conditions (7% CO2) the flux ratio CO2: O2 was close to 4. This was compared with the value expected from the stoichiometries of the linear electron transport chain.
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Tabrizi, H., Schinner, K., Spors, J. et al. Deconvolution of the three components of the photoacoustic signal by curve fitting and the relationship of CO2 uptake to proton fluxes. Photosynthesis Research 57, 101–115 (1998). https://doi.org/10.1023/A:1006025521361
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DOI: https://doi.org/10.1023/A:1006025521361