Abstract
The relation between the fast phase of ms-DLE (delayed light emission measured with a phosphoroscope) and the proton released from water oxidation in spinach chloroplasts was studied in several aspects. When photophosphorylation was allowed to be coupled to the Hill reaction the intensity of the fast phase of ms-DLE of chloroplast was lowered more at 1 °C than at 25 °C, and the photophosphorylation rate within 40 ms of flashing light was higher at 1 °C than at 25 °C. Adding the ε subunit of ATP synthase to the chloroplast preparation to block the leakage of protons through ATP synthase, the intensity of the fast phase of ms-DLE was enhanced, to a larger extent at 1 °C than at 25°C. When the ms-DLE was measured under isotonic conditions, the intensity of fast phase of ms-DLE enhanced by proton released from oxidation of water was more pronounced. The above results support the suggestion that under lower temperature and isotonic conditions, the proton released from water oxidation was liable to be localized and could enhance the intensity of the fast phase of ms-DLE more effectively.
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Albertsson PA (1996) Domains in biological membranes: a study on the organization of the chloroplast thylakoid membrane. In: Moller IM and Brondelius P (eds) Plant Membrane Biology, pp 171-184. Clarendon Press, Oxford
Anderson JM, Goodchild DJ and Thomson WW (1989) The granal margins of plant thylakoid membranes: An important nonappressed domain. In: Baltscheffsky M (ed) Current Research in Photosynthesis, pp II.8.803-807. Kluwer Academic Publishers, Dordrecht, The Netherlands
Bowes JM, Crofts AR and Itoh S (1979) Effect of pH on reactions of the donor side of Photosystem II. Biochim Biophys Acta 547: 336-346
Dilley RA, Theg SM and Bread WA (1987) Membrane-proton interactions in chloroplast bioenergetics: Localized proton domains. Ann Rev Plant Physiol 38: 347
Evans EH and Crofts A (1973) The relationship between delayed fluorescence and the H+ gradient in chloroplast. Biochim Biophys Acta 292: 130-139
Ferguson SJ (1985) Fully delocalised chemiosmotic or localised proton flow pathways in energy coupling? Biochim Biophys Acta 811: 47-95
Junge W (1977) Physical aspects of light harvesting, electron transport and electro-chemical potential generation in photosynthesis of green plants. In: Trebst A and Avron M (eds) Photosynthesis I Encyclopedia of Plant Physiology, pp 59-93. Springer-Verlag, Heidelberg/New York
Lavorel L (1975) Bioenergetics of Photosynthesis, p 223. Academic Press, New York
Malkin S (1977) Delayed luminescence. In: Trebst A and Avron M (eds) Photosynthesis I. Encyclopedia of Plant Physiology, pp 473-491. Springer-Verlag, Heidelberg/New York
Mayne BC and Clayton RK (1966) Luminescence of chlorophyll in spinach chloroplasts induced by acid-base transition. Proc Natl Acad Sci USA 55: 494-497
Nielsen SO and Lehninger AL (1955) Phosphorylation coupled to oxidation of ferrocytochrome C J Biol Chem 215: 555-570
Patrie WJ and McCarty RE (1984) Specific binding of coupling factor 1 lacking the δ and ε subunits to thylakoids. J Biol Chem 259: 11121-11128
Ren HM, Wei JM and Shen YK (1996) On the relationship between the millisecond delayed light emission and localized proton in chloroplast at different temperatures. Acta Phytophys Sinica 22: 296-302
Shen YK (1987) Coupling efficiency and its regulation. In: Fleisher S, King TE and Papa S (eds) Beijing International Symposium on Bioenergetics and Biomembrane, pp 341-357. University Printing Services, TN, USA
Shen YK (1994) Dynamic approaches to the mechanism of photosynthesis. Photosynth Res 39: 1-13
Shen YK and Shen GM (1962) Studies on photophosphorylation II. The 'light intensity effect' and intermediate steps of photophosphorylation. Sci Sin XI: 1097-1105
Shi J, Wei JM and Shen YK (1996) The properties of expressed product of chloroplast atpE gene. Acta Phytophys Sin 22: 225-230
Wraight CA and Crofts AR (1971) Delayed fluorescence and the high-energy state of chloroplasts. Eur J Biochem 19: 386-397
Xu CH and Shen YK (1984) Relationship between the changes of the fast phase of millisecond delayed light emission and the proton released by the oxidation of water. Sci Sin (Series B) XXVII: 37-47
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Wei, JM., Shi, J., Xu, CH. et al. Studies on the relation between the fast phase of millisecond delayed light emission and the proton released from oxidation of water in spinach chloroplast. Photosynthesis Research 57, 317–322 (1998). https://doi.org/10.1023/A:1006011528206
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DOI: https://doi.org/10.1023/A:1006011528206